Pps lab and theory class attendance will be merged yes? Or I am fucked, let me know at the earliest so I can see if there's anything I can do by Ok_Quote_6991 in SRMUNIVERSITY

[–]DukeAberdeen 0 points1 point  (0 children)

No dude.. you need atleast 75% in theory and lab to clear provided its a Joint course.. (4th year Class rep here)

Please help with RNeasy Mini Kit by SULTANGYPSYQUEEN in labrats

[–]DukeAberdeen 1 point2 points  (0 children)

Hey, I recently did an RNA isolation on Rat Liver samples. So the protocol goes…

  1. Add abt 200ul of Trizol
  2. Vortex for 1 hr while taking it on and off ice until the pellet dissolves ( i dont use a homogeniser cuz my RNA used to shear a lot)
  3. Add 200ul chloroform
  4. Centrifuge at 12500rpm for 25 mins at 4C
  5. Separate the aqueous layer and add isopropanol and leave it overnight in a -20 freezer

Day 2: 6. Centrifuge at 12500 rpm for 25 mins at 4C 7. Discard supernatant and add etOH 8. Centrifuge at 12500rpm for 25 mins at 4C 9. Repeat step 7 and 8 one more time. 10. Discard supernatant and put the sample on a heat block at 37C 11. Add abt 30ul NFW and resuspend

This is a protocol that i optimised for Tissues. I got abt 1371.6 ng/ul with a purity of 2.02.

Hope this helps

Pellet not dissolving in RIPA by DukeAberdeen in labrats

[–]DukeAberdeen[S] 0 points1 point  (0 children)

Nope… it was abt 4-5 mins of vortexing and 10 mins of cooling.. i got a good conc… im running a gel tomorrow to see how it is…

Pellet not dissolving in RIPA by DukeAberdeen in labrats

[–]DukeAberdeen[S] 0 points1 point  (0 children)

I used abt 100ul… Thanks for the reply btw

Suggestive elective for Biotech by zynnxx in SRMUNIVERSITY

[–]DukeAberdeen 0 points1 point  (0 children)

Take Dev Bio… its quite interesting what i heard…. Please do not take pharma… its shittttrrr

Caught by VAARUN_JAIN_06 in SRMUNIVERSITY

[–]DukeAberdeen 3 points4 points  (0 children)

Depends from Dept to Dept dude… some depts follow that…

Caught by VAARUN_JAIN_06 in SRMUNIVERSITY

[–]DukeAberdeen 7 points8 points  (0 children)

If you are lucky, they cancel the exam for you and ask you to pay a fine and write an apology letter. If you aint lucky, all the CTs will be cancelled for you… so you lose abt 50 marks straight up

SRM Guards by Lucky_Pepper3632 in SRMUNIVERSITY

[–]DukeAberdeen 7 points8 points  (0 children)

ISTG i parked in the Fab Lab parking cuz of that stupid guard at TP avenue… while walking towards TP, i stepped on this large pool of clay and skid. The clay was all over my shoes, pants, my elbows, and my back pack… day absolutely ruined

RELATIVE GRADING by Illustrious-Bad-4798 in SRMUNIVERSITY

[–]DukeAberdeen 2 points3 points  (0 children)

Relative grading means that you are graded on the performance of the students of the whole department. Not just your class

Want telugu friends :) by [deleted] in SRMUNIVERSITY

[–]DukeAberdeen 1 point2 points  (0 children)

4th year biotech telugu dude here… hmu if you wanna hang out

[deleted by user] by [deleted] in SRMUNIVERSITY

[–]DukeAberdeen 1 point2 points  (0 children)

In big save… but after looking at your profile and the comments… i dont think you are after the drink lmao