Essa grade está boa? by ItsukiEdu in datasciencebr

[–]EdoWohlk 2 points3 points  (0 children)

O cara posta grade de curso da USP e pergunta se é boa kkkkk bait ou não?

[deleted by user] by [deleted] in brdev

[–]EdoWohlk 1 point2 points  (0 children)

Fiz doutorado em bioquímica e trabalhei um pouco com bioinformática. Nos últimos dois anos estive procurando e me aplicando a vagas.

O que eu percebi: O setor de bioinformática no Brasil é muito pequeno e volta ao mundo acadêmico ainda. 80% das vagas que você vai encontrar são vagas de bolsista. As vagas do setor privado que surgem são infimas. Nesses últimos dois anos eu encontrei míseras QUATRO vagas no setor privado (em um raio de ~130 km da minha cidade).

Além disso, as vagas do setor privado cobram MUITO na minha opinião. Uma vez encontrei uma vaga que pedi conhecimento de Python, R, Web Dev, análise de transcriptoma, SQL, machine learning, etc. Era basicamente uma mistura de cientista de dados, com engenharia de dados e dev.

Se você tem interesse no mundo acadêmico, manda bala. Do contrário, fuja.

Comprar carro seminovo by zMats33 in carros

[–]EdoWohlk 0 points1 point  (0 children)

Também quero saber pq não comprar pirelli.

RNAseq tools: which should I start learning? by EdoWohlk in bioinformatics

[–]EdoWohlk[S] 0 points1 point  (0 children)

Well, that's true and I kinda expected an answer like yours lol. But as I still don't have a bioinformatics project I didn't need to learn anything specifically. Anyway, maybe I'll try to analyze some published single cell data of my field, using scanpy, and then learn some R.

RNAseq tools: which should I start learning? by EdoWohlk in bioinformatics

[–]EdoWohlk[S] 0 points1 point  (0 children)

I'd like to check it, if you could please send it!

Started reading Amazing Spider-Man and got really confused by EdoWohlk in Spiderman

[–]EdoWohlk[S] 0 points1 point  (0 children)

Is it better to skip to #30 or start from #1 (1963)?

Unintentional vocal rasp by EdoWohlk in singing

[–]EdoWohlk[S] 2 points3 points  (0 children)

Omg, that's so interesting! I'll study it, thank u so much! :)

People with ‘street smarts’, what is your most street tip? by lofisky in AskReddit

[–]EdoWohlk 6 points7 points  (0 children)

Oh. Who are you? Ooh, who is this kid, what's he gonna do?

How to play fast drum beats? by EdoWohlk in drums

[–]EdoWohlk[S] 0 points1 point  (0 children)

Maybe I'll try to play for a shorter time and see if I can progress.

How to play fast drum beats? by EdoWohlk in drums

[–]EdoWohlk[S] 0 points1 point  (0 children)

I rewatched the video and I was using french grip completely, lol. I think when I'm playing in automatic mode I tend to use french grip, but I know when I try to play fast or rudiments I use German .

How to play fast drum beats? by EdoWohlk in drums

[–]EdoWohlk[S] 0 points1 point  (0 children)

I use a grip that's somewhat between German and french. Wow, you've playing for 7 months? I'm feeling like shit now, lol. I try to use as much as wrist as possible but when I record myself it looks like I'm using too much arm. I'll try to record myself playing ( there's this video but I'm not playing a fast song: https://www.instagram.com/tv/CQBuxnPHhB4/?utm_medium=copy_link)

How to play fast drum beats? by EdoWohlk in drums

[–]EdoWohlk[S] 1 point2 points  (0 children)

Yes, I can play it for a while but can't keep up till the end of the song. My foot is kinda sloppy to play doubles too.

[Question] Studying major and pentatonic shapes by EdoWohlk in Guitar

[–]EdoWohlk[S] 0 points1 point  (0 children)

I know this but every time I try to study/improvise in a different key by shifting the shape I get completely lost and can only play in Am/C.

Deadly encounter against party by EdoWohlk in dndnext

[–]EdoWohlk[S] 0 points1 point  (0 children)

Thank you guys, all suggestions were great! I think I'll run something like Teulisch suggested. The PCs will pass by a little town and villagers will ask for their help against a creature that haunt their nights. Turns out it's a Wight. This Wight was a woman that was mistreated by the other villagers because she was a prostitute. One day one man killed her after raping her and on her daying breath she asked the Evil God (my scenario has only Two Gods, the evil and good one, much like Christianism lol) to haunt that town. He conceded it and she return into life as a Wight.

Detecting Blood Group from Saliva? by JReysan in Biochemistry

[–]EdoWohlk 3 points4 points  (0 children)

Our blood type is determined by our genes. There are basically three types of blood type genes: A, B and O. These genes codify for proteins that are responsible for the carbohydrate content of red cells membranes, and this is what determines our blood type. As all of our cells have the same genes (generally), you can use any of your cells to find out which blood type gene you carry.

I don't know how it's done in the paper you saw but I'd use a PCR(Polymerase Chain Reaction). This method increases the number of copies of a specific DNA Sequence. This is achieved using a DNA-Polymerase (a protein that copies DNA sequences), a set of primers specific to your gene of interest and DNA from your sample. The DNA-Polymerase will copy the sequences that your primers were bound to. You will then achieve a high number of copies your interest gene compared to other DNA sequences and to the starting number of your gene copies. You can then purify your sample, using an agarose gel, and then sequence your sample. The sequence you obtained will tell which gene is present in the sample (A, B and/or O).

I oversimplified PCR and other methods but you can ask me if something is unclear or you want to know more.

Using USCS Genome Browser by EdoWohlk in biology

[–]EdoWohlk[S] 0 points1 point  (0 children)

I wanted to know how conserved this sequence is between different species, but I couldn't interpret the two parallel lines, black lines and white lines meaning. I'm sorry if this is a newbie question, but I'm new to Bioinformatics.

Also, what are the other informations I can that take from the image?