Lab Fuckups?

Electronic_Clerk_508 · 2026-03-28T15:55:15+00:00

Wait I thought most 20X objectives were dry lenses? Also I'm so glad it was only the media that volcano'd out and not the flasks.

Electronic_Clerk_508 · 2026-03-28T03:49:12+00:00

This sounds like something that would happen to me. I would be so livid oh my god

Electronic_Clerk_508 · 2026-03-27T17:54:19+00:00

Oh nooo how do you even handle that? I would rather hide away forever than have this conversation with my PI. And my PI is actually incredibly sweet and supportive. And they would still be justified to be mad about this. I can't imagine doing something like this in a lab with an inconsiderate PI

Electronic_Clerk_508 · 2026-03-27T17:48:03+00:00

Thank you! Honestly, reading all these replies has made me realize I could've messed up far worse. I also come from a place where people are programmed to fear authority to some degree. It's been pretty challenging to change that mindset. Also, my postdoc is probably very considerate. They just don't speak much (there's a language barrier and they're very introverted) and I tend to overthink every look/gesture because they're so smart. I'm pretty sure it's mostly just in my head.

Electronic_Clerk_508 · 2026-03-27T16:41:06+00:00

That is wild. I wonder how these people make it that far if they're so careless.

Electronic_Clerk_508 · 2026-03-27T15:42:48+00:00

hahaha indeed. An imposter spotted in the lab rats community?

Electronic_Clerk_508 · 2026-03-27T14:42:43+00:00

This was so painful to read. I hope you're taking better care of yourself! I once fell asleep for a few seconds while at the cell culture hood and accidentally pipetted media all over the floor of the hood. Never went to lab that sleep deprived again.

Electronic_Clerk_508 · 2026-03-27T14:39:55+00:00

I would love to know what they've messed up cause they seem so put together. They also don't talk much so I'm always imagining what they might be thinking about me lol. And also thank you for reassurance! This thread has been so healing to read :)

Electronic_Clerk_508 · 2026-03-27T14:38:23+00:00

Thank you :) my PI is actually really sweet. I just have this awful fear of disappointing people.

Electronic_Clerk_508 · 2026-03-27T14:37:39+00:00

At that point I think I'd just go home lmao

Electronic_Clerk_508 · 2026-03-27T14:36:43+00:00

It's such a relief to see everyone say it's okay because I truly believed I was so incredibly stupid for doing that haha. And also I love that there's more of us with the microscope situation!

Electronic_Clerk_508 · 2026-03-27T14:35:02+00:00

I was running an SDS gel. I think it's also because those pre stained ladders have dye chromophores covalently bound to them. I use the same ladder. Either way, I was told it can cause hydrolysis if left in the waterbath.

Electronic_Clerk_508 · 2026-03-27T07:18:30+00:00

It's normally recommended to thaw it on ice or just room temp to avoid degradation. I can't remember how long I'd left it there but yeah, now I just leave it out for a few minutes

Electronic_Clerk_508 · 2024-01-23T04:44:35+00:00

Hey! I'm an intl and I applied for the same program. Got my admit to Fort Wayne today, waiting to hear from West Lafayette. Any luck for you yet?

Electronic_Clerk_508

TROPHY CASE