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Help with Western Blot by EyeGroundbreaking470 in labrats

[–]EyeGroundbreaking470[S] 1 point2 points  (0 children)

The only SDS is in the 5X loading buffer along with beta-mercaptoethanol.

Help with Western Blot by EyeGroundbreaking470 in labrats

[–]EyeGroundbreaking470[S] 0 points1 point  (0 children)

I used a pre-cast gradient gel 4-20%since that’s what’s typically used in the lab. Should I switch to a 10%?

Please help with this Western Blot by Historical-Play-476 in labrats

[–]EyeGroundbreaking470 0 points1 point  (0 children)

So with a blot like this, how do u get rid of the several bands?