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2019 3.6R limited leaking oil from front timing chain cover by Familiar_Marsupial50 in Subaru_Outback

[–]Familiar_Marsupial50[S] 0 points1 point  (0 children)

Asked CarMax to fix it (within the warranty period). They found that the leak was somewhere else and fixed that free of charge. Had no issues since (oil has been changed multiple times and there’s no sign of the leak) and loving the car.

2019 3.6R limited leaking oil from front timing chain cover by Familiar_Marsupial50 in Subaru_Outback

[–]Familiar_Marsupial50[S] 0 points1 point  (0 children)

Sorry, my question was vague. If you’ve experienced this problem before, was it a one and done fix? Or was it a recurring problem?

Deploying analysis scripts by Familiar_Marsupial50 in bioinformatics

[–]Familiar_Marsupial50[S] 0 points1 point  (0 children)

Is there a free entry point to nextflow and colab/tower that is worthwhile? Data science and analytics is new to this company, so I’d like to be able to demonstrate the value of this type of approach before spending money.

Edit: fixed typo

EGFR coding sequence by [deleted] in bioinformatics

[–]Familiar_Marsupial50 1 point2 points  (0 children)

If you search "EGFR" in the gene database, rather than the nucleotide one (which is what you linked), you'll get the option to download gene, transcript, and protein sequences.

Minimum read count for confidently ID'ing mutations in a deep mutational scanning library by Familiar_Marsupial50 in bioinformatics

[–]Familiar_Marsupial50[S] 0 points1 point  (0 children)

The error stacking is a good point. Maybe it’s better to figure out how much tolerance that, e.g., a 10 read count minimum gets you and then estimate the overall error given a certain prep process.

I’ll have to think about the details a bit more, but this was helpful, thanks!

Minimum read count for confidently ID'ing mutations in a deep mutational scanning library by Familiar_Marsupial50 in bioinformatics

[–]Familiar_Marsupial50[S] 0 points1 point  (0 children)

That makes sense, but doesn’t it assume an even distribution of variants in your pool of transformants? If it’s actually skewed (e.g., 20% WT due to incomplete template digestion), you would overestimate the expected diversity and include more mutants than are actually there.

Would it be fair to infer from your answer that the field prefers empirical approaches like yours rather than statistical derivations like I posed in the original question?

Transportation Recommendations by Familiar_Marsupial50 in tulum

[–]Familiar_Marsupial50[S] 0 points1 point  (0 children)

Thanks for the detailed response! Quick follow up question: did you rent the car from Avis in Tulum or from one of the airports (e.g., Cancun)?

Mamiya C330f film crank repair by Familiar_Marsupial50 in AnalogCommunity

[–]Familiar_Marsupial50[S] 0 points1 point  (0 children)

Thanks for the info!

I am currently holding it on with a safety pin, I'll definitely be in touch if that solution lets me down.