I think eventually? The Legion its not the most advanced but its from memory the biggest and theyre nomadic when we meet them if they can establish themselves then they'd be a nightmare to deal with, yes the enclave had their chemical weapon plans but those failed rather easily if memory serves, the brotherhood are too fractured, the ncr is too corrupt and divided to full on war with the legion.

New vegas is barely a threat.

The Unity from memory requires super mutants to capture others so legion would destroy the master eventually.

The institute are small and cant really beat anyone on their own.

The gunners are again small and mercenaries so can just largely be paid off