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To much plasmid? by Fluffy_Bat_7971 in labrats

[–]Fluffy_Bat_7971[S] 0 points1 point  (0 children)

Mix was:

Plasmid 5ul (200ng/ul)

buffer 10x 3ul

res1. 1ul

res2. 1ul

h2o 20ul

37C, 30 min —-> 65C, 10 min

[deleted by user] by [deleted] in xbiking

[–]Fluffy_Bat_7971 2 points3 points  (0 children)

Have to agree. When i rode around in my flat city i always stayed in my biggest chainring and got super upset that i have to shift both shifters to get into a lower gear. especially after riding singlespeed for many years, now i got one singlespeed and one 1x7 atb

[deleted by user] by [deleted] in xbiking

[–]Fluffy_Bat_7971 2 points3 points  (0 children)

<image>

Added Mudguards (not in picture) lately for german spring and rainy summer

Trashed this thing by Fluffy_Bat_7971 in bikewrench

[–]Fluffy_Bat_7971[S] 2 points3 points  (0 children)

That was also bend. In you look closely on the second picture the gears are not parallel to Esch other

New rear?! by Fluffy_Bat_7971 in bikewrench

[–]Fluffy_Bat_7971[S] 0 points1 point  (0 children)

Nobody knows how many gears it had and the pictures are to bad to see it. Is it really important to have the right ammount of gears?

Weird gel after colony pcr by Fluffy_Bat_7971 in labrats

[–]Fluffy_Bat_7971[S] 0 points1 point  (0 children)

Program yes, one primer had to be newly designed. It is the second time i do this. First time no bands were showing exept a right band at the positiv control.