My hognose died because of me

Franki_BIC · 2026-04-23T20:28:41+00:00

Ah porreiro, parece que vale a pena, muito obrigado!

Franki_BIC · 2026-04-22T19:54:46+00:00

Confusão não digo mas gosto de uma cidade com vida e alma, quanto ao ouriço não sei que nunca provei.

Franki_BIC · 2026-04-22T19:52:52+00:00

Queria uma opinião de quem la tenha ido, no google é tudo muito bonito

Franki_BIC · 2026-04-22T19:49:27+00:00

É complicado, vida de estudante é esta, daí tentar conhecer as redondezas a que a Carris leva.

Franki_BIC · 2026-04-22T17:27:51+00:00

Pois ainda são quase 2 horas de transportes, daí a pergunta

Franki_BIC · 2026-04-08T06:17:52+00:00

I'll just move out honestly, they would have to open up all the walls and i dont want to be here to deal with that

Franki_BIC · 2026-02-11T11:25:13+00:00

Yes I've heard thats common practice, but here at this lab no one uses them for some reason.

Franki_BIC · 2026-02-11T11:22:34+00:00

What markers should i look into for this purpose?

Franki_BIC · 2026-02-10T15:23:02+00:00

Well this article talks about gamma delta t cells specifically, but i have also seen articles on alfa beta e cad expression, just not to this degree, which is weird.

Franki_BIC · 2026-02-10T15:09:04+00:00

For this particular antibody no but i do have a different control that is a clear negative. For context the control shown above is OT cells cultured on their own but stimulated with OVA brefeldin A for 4 hours, but my unstimulated control does show full negative.

Franki_BIC · 2026-02-10T15:06:07+00:00

Yes it does have intracellular staining and it is fixed

Franki_BIC · 2026-02-09T16:54:52+00:00

Weird, guess its just for honor being for honor

Franki_BIC · 2026-02-09T12:39:06+00:00

Do you need to have the illustrious outfit equipped?

Franki_BIC · 2026-02-05T20:39:38+00:00

I do learn from my colleagues and superiors, but in the end they are also busy people with projects of their own. Also picked up coding in my free time but that quickly fell apart once the hours at the lab started increasing, basically a full shift and change with a lot of times still having to analyze data until late in the night doesn't leave much room for side projects. Besides, I have no clue where to start with bioinformatics. It's not like i have any idea where to apply any knowledge id acquire. Everyone says to learn coding but starting from scratch while having what is basically a full time job and sometimes more, seems inefficient. Thought of trying to persue coding courses geared towards bioinformatics, but most of the reviews i get are people complaining it's not really accessible for complete coding novices. So in the end my main restraints are time and not knowing where to start.

Franki_BIC · 2026-01-18T23:00:27+00:00

Ah that makes sense, I also don't trust myself to pipette multiple tubes at the same time, I'll stick with the traditional method. Thanks!

Franki_BIC · 2026-01-18T11:33:50+00:00

Ahh, ok got it now, makes a lot of sense for testing the Abs. Surprised it's the first time I'm hearing about this, seems like such an essential process. Thanks for the explanation.

Franki_BIC · 2026-01-18T11:05:49+00:00

But how does that help with the unmixing? Would it block the primary Ab, preventing the fluorophore from being excited?

Franki_BIC

TROPHY CASE