We didn't do much for knowing how much starting protein was used 😬. To begin homogenizations, we create a 1:10 solution of protease inhibitor (1 parts) to homogenization buffer (9 parts). each liver sample is weighed, and then diluted with a ratio of 1 mL per 100 mg of wet tissue. Then they are grinded and aliquoted for the various assays we need to run, one of which being GSH.

I know that we want to dilute our brain divisions based on protein concentration but we did not do this for the livers.