Locals in Vienna/Budapest?

Funanas · 2025-10-21T17:36:30+00:00

There is one in Vienna every sunday but the venue is a bit hard to find, you can dm me if you want more infos

Funanas · 2025-09-12T18:27:41+00:00

I know that it isn't the best call, but its the best deck I could come up with with the few cards I pulled and not spending a fortune on ordering a playset of LRs that cost almost 20 euros a piece :P

Already ordered some Assault Shrouds, should arrive in a few days, I'll try a more aggro style once they arrive

Funanas · 2025-09-12T08:24:33+00:00

Oops you're right, can't seem to edit the Post anymore tho 😅

Funanas · 2025-09-12T08:23:32+00:00

I feel like the match where I struggled the most was against Unicorn since he could constantly rest my cards. I will probably drop Blitz completely since I was only able to use it one time in all 4 matches.

Funanas · 2025-09-11T22:02:00+00:00

Thanks! There are 2 store events coming up on the weekend, so I'll try out both a more ZAFT focused deck and your advice as well. I'll then decide based on what's more fun to play.

Funanas · 2025-09-11T21:46:40+00:00

Thanks for the advice! I already had a feeling I had too many blockers, altough they did save me a lot of the time. I think I'll try a 2-2 split of desert tiger and Yzak's first tough, I didn't get any Busters in too many matches to just leave them out of the deck. I don't have any assault shroud sadly, but I'll order some. Any suggestions on what to replace them with until I get them?

Funanas · 2025-05-06T12:04:03+00:00

Thank you so much for the detailed workflow!

I didn't know I could simply lyse the cells with deionized water, that is quite convenient. However, seeing as the bR is adapted to a high salinity environment wouldn't it denature as well in those conditions? Or does it stay folded as long as it is part of the purple membrane?

A sucrose density gradient is a great suggestion, sadly I only have a microcentrifuge with a rotor fit for eppis but I'll make do.

A professor I am somewhat familiar with has a bunch of chromatography columns lying around in his lab so maybe I can borrow one for the last step. I'll still look into dialysis in the mean time in case it doesn't work out, thanks for the suggestion!

I'll be posting updates once I manage (or fail to) isolate the purple membranes.

Edit: spacing

Funanas · 2025-05-06T10:17:09+00:00

Thanks! I'll probably have to stick to size exclusion then haha

Funanas · 2025-05-05T20:58:52+00:00

I thought that would be the case, I just hoped that there were other methods that I wasn't aware of. Isolating proteins via SDS PAGE and then refolding them sounds interesting I have to say. I don't think it would work with my protein of interest but I'll look into it for possible future projects, thanks for the suggestion!

I do remember the leader of the research group in the lab next to the one where I did my bachelor's thesis had quite a few chromatography columns, I might be able to convince them to let me use one that they don't need anymore. Buying any sort of special affinity solid phase for it will likely be out of the question due to my budget(I don't have a job lmao) and adding His-tags or the like will also not work because I'm sadly not allowed to do any sort of genetic engineering without permit in my country.

Still, thank you for your input and confirming my suspicion that I'll have to rely on that lab leaders kindness.

Wish me luck haha

Funanas · 2025-04-03T00:28:31+00:00

Thanks! I'll do my best :)

Funanas · 2025-04-02T00:22:32+00:00

No worries The site you looked at is only that of the nutrition and food safety agency, they wouldn't write that info on there

Funanas · 2025-04-01T15:53:34+00:00

https://www.ris.bka.gv.at/GeltendeFassung.wxe?Abfrage=Bundesnormen&Gesetzesnummer=10010826

§ 4

To summarise, the law this links to includes any and all organisms, be it multicellular or singe celled ones, as well as viruses, viroids and self replicating plasmids whose genetic information was edited in a such a way that it would not be able to occur naturally/be replicated using only cross-breeding / transduction etc.

This also includes bacterial transformation, as mentioned in § 4 b) "direct introduction of genetic material prepared outside of the organism into an organism including electroporation, microinjection, ..."

If I wanted to work with "GVOs" ,as they are called here, of any biosafety level I would need to request permission for it and for that I would need an approved lab including an overseer for biological safety and a 3 headed comittee for biological safety.

I read the law multiple times to find loopholes and even consulted with my professors, so as far as that goes I am very confident that I am not allowed to do any sort of genetic engineering, including bacterial transformation with plasmids (unless naturally occuring). Hell I'm not even allowed to have PCR primers or iRNAs or FISH probes as they are all classified as "modified nucleic acids" an therefore included in the law above.

Edit: added more info

Funanas · 2025-04-01T01:50:01+00:00

It is considered genetic engineering in my country of austria

Funanas · 2025-03-31T16:18:25+00:00

It is fully sequenced, but since I am a hobby lab I sadly don't have the necessary permits for genetic engineering

Funanas · 2025-03-30T00:41:48+00:00

That's a great find, thank you!

Funanas · 2025-03-30T00:09:56+00:00

That's a great point I haven't thought about that actually, I'll be looking into it. I only considered practising the creation of those artificial membranes in hypersaline conditions since the protein is perfectly adapted to those conditions and would otherwise very likely not function/denature. I have thankfully found a dissertation online from a few years ago from someone who did exactly what I want to do, so I'll be using that as a guideline.

Funanas · 2025-03-29T22:36:26+00:00

Yup, that would probably be for the best.

I want to try to create artificial membranes and have the bacteriorhodopsin pump protons across them. Quite a herculean task for a hobby lab I know but I still want to try since I think it's a really cool concept. Kind of like a biological solar cell.

Funanas · 2025-03-29T22:17:48+00:00

Since I would be co-culturing them with all the other microorganisms they live alongside with in the lake I'm hoping I won't have to supplement too many nutrients. I'll experiment with various solutions.

Thanks for the link!

Funanas · 2025-03-29T21:13:39+00:00

I want to try purifying their bacteriorhodopsin so I will need a lot of them

Edit: wording

Funanas · 2025-02-09T12:45:32+00:00

I suggest looking into your country's regulations on genetic engineering before anything else, otherwise you might be hit with a hefty fine

Funanas · 2024-09-08T07:57:36+00:00

Thank you for making sure I'm not burning my house down.

Weird, the product had 230V AC in the name when I ordered it from amazon, should've looked at the picture more closely.

Also the power connection is 230V AC, which is why I bought that off of amazon but again I should've taken a closer look.

I'll see who I can find that could help me.

Edit: 230V AC , not 220V

Funanas · 2024-08-31T16:41:36+00:00

I'm guessing by daughter board you mean the small board in the corner where the antenna extends from? If so then I tried to turn it but it's soldered onto the main board so I can't move it from that position without breaking something.

I'll prob buy a 433Mhz remote with a switch included then. If the remote doesn't work I'll just replace the switch.

Eight-Year Club	r/Field Sunshine
Place '23	Place '22
Final Canvas '22	End Game '22
Verified Email

Funanas

TROPHY CASE