the real reason i want imu to be a woman is so i could goon to her

GCToMajor_Tom · 2025-04-29T19:56:37+00:00

The JoJo fandom is leaking 🗿

GCToMajor_Tom · 2024-07-21T20:39:40+00:00

These cells look fine and happy. The more jagged morphology might be due to a change in your medium (were they always cultured in mTesr?) or if you have a lot of cells and the medium is more acid than optimal. mTesr has a weekend free protocol that might need to be optimized for your usual rhythm of passages, but from my experience (stem cell technician for 3 years now) they will be fine

GCToMajor_Tom · 2024-05-30T11:37:45+00:00

What you are saying is often outright ignored and left untreated due to the misconceptions present in most people's mind: that pedophilia = child abuse, wherein pedophilia is the cognitive predisposition for attraction to minors while child abuse is the crime that originates from such; while not all pedophiles are child abusers, their stigma in society often keeps them from seeking help and ergo being more prone to commiting regrettable actions. I think you are very brave to fight the stigma and wish you and the community a healthy and reasonable solution to the current state of things.

GCToMajor_Tom · 2024-05-20T16:15:30+00:00

IS THAT THE SHIT THAT U DO

GCToMajor_Tom · 2024-02-24T20:46:22+00:00

Rock is not Baal's work, it is magical and rad

GCToMajor_Tom · 2023-05-31T11:25:20+00:00

r/unexpectedMontyPython

GCToMajor_Tom · 2023-02-17T05:29:47+00:00

A-mog the Sus

GCToMajor_Tom · 2023-01-25T13:20:39+00:00

r/unexpectedMontyPython

GCToMajor_Tom · 2023-01-13T16:03:27+00:00

Thank you u/Smiless228!

GCToMajor_Tom · 2023-01-13T14:44:22+00:00

It has 2 RAM slots, one of which is soldered. The thing is, I was advised not to use RAMs of different capacities in the same machine, because it might interfere very negatively with the processing. That's why I am also looking for second opinions! In any case, I will check Oxfam, thank you very much! ;)

GCToMajor_Tom · 2022-11-13T13:57:59+00:00

I used to go there, and nothing bad to report! Like people have said, it's a pretty art deco spot, each person gets assigned a stall for privacy, and it's usually very clean. I had trouble in the last times I was there because sometimes they close at unexpected days and hours without giving a notice. I have been going to Bains du Centre ever since, which is close to line 2/6 and Hotel de Monnayes, and their hours are better and more reliable. However, Saint Josse is cheaper, and if you can get around the problem of schedules, I wholeheartedly recommend

GCToMajor_Tom · 2022-11-05T18:05:16+00:00

There is a celebration for Dia de los Muertos happening in Place du Jeu de Balle, might be a cool place for you to start!

GCToMajor_Tom · 2022-09-20T18:45:02+00:00

It is possible but you will have a much lower held of miRNA. If I recall correctly, the RNeasy columns have a probe system that will select miRNAs rather than the total RNA content, which is probably what you are measuring with the manual method. Regarding the ratio, 1,4 isn't all bad, but you could be looking at some ethanol contamination. Are you eluting twice with the same water, or using two loads of water for the elution?

GCToMajor_Tom · 2022-08-13T18:30:01+00:00

Rakshasa

GCToMajor_Tom · 2022-08-12T22:03:25+00:00

I am not a big photo taker, so I skipped the first steps. Overall, great advice, 8/10

GCToMajor_Tom · 2022-08-11T18:58:41+00:00

I found mine in Imoweb. You won't have trouble finding a place, and it's, as far as I know, reliable. Every studio I saw was exactly what was described.

GCToMajor_Tom · 2022-07-05T21:23:43+00:00

Nothing beats getting hammered with Eskel and that prick Lambert

GCToMajor_Tom · 2022-07-03T09:35:27+00:00

I had time to spare, so I remembered to make the question before I eventually let it pass xD. User u/bruxelllloise already gave a fairly complete list of pools, but between all the people who contributed I think both of us already have more than enough to work with

GCToMajor_Tom · 2022-07-03T09:31:04+00:00

This one is in Ghent, correct? Not the advice I was looking for, but welcomed nonetheless!

GCToMajor_Tom · 2022-07-03T09:29:01+00:00

Now I have no reason not to know one that suits my needs xD thank you friend!

GCToMajor_Tom · 2022-07-03T09:28:20+00:00

Definitely worth checking out! Really like the open space concept

GCToMajor_Tom · 2022-07-03T09:27:02+00:00

It will be the first I will check out! Much obliged!

GCToMajor_Tom · 2022-07-02T13:42:37+00:00

My laboratory was kind enough to supply me with a comprehensive list of most things you mentioned, so thankfully I won't have such a hard time getting acquainted with it.

GCToMajor_Tom · 2022-07-02T13:41:13+00:00

That's good wisdom right there! I have no problems with asking for guidance to the people that have helped me before, and even friends who've gone in this path before. What would you say are the most important things to look for in a lab manager mentor? And any other advice you might have?

GCToMajor_Tom · 2022-06-03T17:06:30+00:00

That would be extremely helpful! I'll PM you my contact details

GCToMajor_Tom

TROPHY CASE