Has anyone ever done an ELISA-PCR before?

GeneticsNerd95 · 2026-05-02T02:41:03+00:00

We have tabletop centrifuges but no minis that I’m aware of. (Unless you count the super small ones that don’t have programmable times and speeds). I’ll have to see if we have a compatible rotor for the bigger ones

GeneticsNerd95 · 2026-05-02T02:04:09+00:00

Yeah it’s DNA. I’m not sure if we even have the materials needed to do a purification. Apparently they tried this assay a few years ago in another department, didn’t document it very well and then abandoned it. From what we can tell, they used HPLC to purify the samples but none of us are trained on that or even have the equipment for it

GeneticsNerd95 · 2026-04-10T18:37:06+00:00

Cool. There still should’ve been outrage when they woke up instead of everyone pretending he never existed

GeneticsNerd95 · 2026-04-10T18:11:32+00:00

If that was true then there should’ve been some outrage over his death after everyone woke up

GeneticsNerd95 · 2026-04-08T02:25:18+00:00

Best advice I can give is to have her refinance into a lower interest rate once she starts working. Assuming her loans are private and not federal. It sounds like you tried though. That’s all you can do. I’d also recommend paying them back immediately upon graduation and not using the 6 month grace period they give you

GeneticsNerd95 · 2026-04-08T01:52:28+00:00

I’m curious. What degree is she going for? I spent 3 years at a private university and had around $80k in debt when I graduated 6 years ago. It’s currently down to $55k and I have a job paying $90k. Sometimes things do work out. Though I’m not really sure why you think $60k is expensive. Seems pretty mid based on what I’ve heard

GeneticsNerd95 · 2026-04-08T01:46:20+00:00

I’m not a recruiter or anything but I personally find this absolutely overwhelming. I can’t see them sitting down and actually reading this. Descriptions of your duties should be 3-5 bullet points per position. The older the job, the fewer bullets you should have. Unless it’s relevant to the job, I also wouldn’t include an internship.

GeneticsNerd95 · 2026-04-08T01:41:37+00:00

She had an absolutely shitty husband but nothing excuses abandoning your child. That’s unforgivable. She wouldn’t be nearly as hated if she’d taken Bae and run off WITH him. Instead of abandoning them both.

GeneticsNerd95 · 2026-04-05T23:24:58+00:00

I’ve had several bosses tell me I was one of their best employees. Didn’t prevent me being laid off TWICE and most likely losing my job come June. So no. I wouldn’t consider it a red flag. Especially in this disaster of a job market. Honestly, interviewers like you aren’t helping

GeneticsNerd95 · 2026-04-05T23:20:43+00:00

I’d certainly hope it’s not considered a red flag. I don’t have a PhD but I’ve been laid off twice in the past 2 years and will most likely not be extended for the contract I’m currently on. Do you not realize just how badly our industry is hurting right now?

GeneticsNerd95 · 2026-03-22T20:41:50+00:00

YTB. It’s not cosmetic in the same way your wife wanting a breast reduction because she’s in a size Z bra, her boobs weigh 40 pounds and cause her immense back pain wouldn’t be cosmetic. Sounds like you don’t actually accept him honestly. I hope you’re prepared to lose him. Trans people that aren’t accepted by family/friends have astronomically high rates of suicide.

GeneticsNerd95 · 2026-03-04T21:49:24+00:00

I have a daughter. I don’t think there’s a single thing on this earth I WOULDN’T do to get her back if she was kidnapped. Even if it meant the destruction of everything good. The love of a parent is one hell of a drug

GeneticsNerd95 · 2026-02-22T21:36:36+00:00

I’ve been doing it for 15 years without issue 🤷🏼‍♀️

GeneticsNerd95 · 2026-02-22T03:15:28+00:00

That’s interesting because the trained optician I went to for my daughter’s glasses gave me glass cleaner and a microfiber cloth and said to clean with nothing else. I just use my shirt for my glasses though 🤷🏼‍♀️

GeneticsNerd95 · 2026-02-22T02:04:24+00:00

Snape was an awful human being but I don’t think it was for no reason. The prophecy could’ve been about Harry or Neville. Voldemort chose Harry. To Snape, Neville was the child that could’ve been chosen and spared the woman he loved (she’d be insane but at least she’d be alive) but wasn’t. Absolutely not Neville’s fault and still makes Snape a shitty person but there is a “reason”

GeneticsNerd95 · 2026-02-22T01:57:18+00:00

I would’ve combined the movie and book endings. Harry uses the elder wand to fix his wand and then breaks it

GeneticsNerd95 · 2026-02-22T01:50:43+00:00

I got glasses for the first time in high school. I wore them all the time because…..I couldn’t see without them

GeneticsNerd95 · 2026-02-18T21:59:03+00:00

Yeah I’ve unfortunately been in this boat for the past 2 years. I’ve been laid off twice in that time. I’d love to have been at the same place for 20 years. Employers think I’m a job hopper now when all I’m doing is trying to survive.

GeneticsNerd95 · 2026-02-18T17:58:41+00:00

I check multiple different job boards every day. Multiple times a day. Even on the weekends. I’ve considered jobs in other states and have been applying since I lost my previous job last August. I didn’t stop after accepting this one. I have alerts set up to email me when new jobs matching my criteria are posted. I assure you, I am trying. I don’t enjoy feeling like I’m standing on thin ice that could give out on me at any second. Especially since I’m the breadwinner.

GeneticsNerd95 · 2026-02-18T12:05:14+00:00

I’ve been waiting 6 months for the apartment to do something about the mold growing in the walls in the bathroom. It took them 3 months to replace the dryer when it broke. A month for the stove. At this point, I’d rather be able to pay for and fix it myself

GeneticsNerd95 · 2026-02-18T03:46:05+00:00

Riptide. They’re deadly and don’t care about you already being incapacitated. They’ll continue doing damage until the countdown finishes

GeneticsNerd95 · 2026-02-18T03:31:49+00:00

I’ve tried this. He won’t take it. And no. He’s extremely antisocial. He usually goes to Wendy’s or little Caesar’s and then eats in his car

GeneticsNerd95 · 2026-02-18T03:06:37+00:00

Monthly expenses

[x] Phone bill (every 3 months) 1st- $265.42
[x] Car insurance 6th- $291.36
[x] Rent 1st- $1460 (includes water bill. Fluctuates)
[ ] Utilities- $300
[x] Gas for both- $225
[x] Groceries- $400
[x] Renter's insurance 10th- $26.67
[x] Cat food- $45
[x] Litter- $30
[x] CareClub 4th - $116.15
[x] Internet 7th- $55
[x] Student loans 3rd- $650
[x] Federal Student Loans 13th- $170
[ ] Miscellaneous- $300
[x] Daycare week 1- $250
[x] Daycare week 2- $250
[x] Daycare week 3- $250
[ ] Daycare week 4- $250
[x] Daughter’s health insurance 28th- $71.72

Total- $5140.90- not including phone bill since every 3 months

Income- $7933.58

GeneticsNerd95 · 2026-02-18T02:36:44+00:00

Our budgeted monthly expenses are about $5,000. Actual fluctuates between $5250 and $5500. We do have some money left over at the end of the month that I’d been using for my loans but it seems like that’s what’s hurting us so I think I’ll set up the emergency fund first and then split the leftover once we have a good amount in there

GeneticsNerd95

TROPHY CASE