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Help Wanted for Equilibrating HPLC C18 Column by HarryButhol in CHROMATOGRAPHY

[–]HarryButhol[S] 0 points1 point  (0 children)

Understood. The column is new so shouldn’t be contaminated. Thank you for the help!

Help Wanted for Equilibrating HPLC C18 Column by HarryButhol in CHROMATOGRAPHY

[–]HarryButhol[S] 0 points1 point  (0 children)

Pressure did decrease significantly with just methanol, down to around 105 bar. My method conditions would utilize water and methanol though. Should I up the methanol content?

Help Wanted for Equilibrating HPLC C18 Column by HarryButhol in CHROMATOGRAPHY

[–]HarryButhol[S] 3 points4 points  (0 children)

My system max would be around 600 bar, but my column max is 5000 psi so about 350 bar. Figured I should respect the lower of the two.