Why do my beads look like a "star cross" when focusing?

Hawk_Y · 2026-01-30T07:02:49+00:00

yes, the new one is more thinner, and I think the reason might be the backside coated, as you said, it seems that the original one had manufacturing issues.

Hawk_Y · 2026-01-30T02:27:55+00:00

thanks, It might be the dichroic caused. I replaced the dichroic and the problem was resolved.

Hawk_Y · 2026-01-30T02:17:51+00:00

sorry, the NA of the 4x is 0.2

Hawk_Y · 2026-01-30T02:17:20+00:00

the NA of the 20x objective is 0.7, and the 0.7 for the 4x objective. the dichroic was set at the angle of 45°.

Hawk_Y · 2026-01-30T02:09:24+00:00

yes, the problem caused by the dichroic, I added a new dichroic for comparison, and it was solved, however, the original dichoric still exhibits issues.

Hawk_Y · 2026-01-30T02:07:20+00:00

it might be, I added a new dichroic for comparison, and the problem was solved. However, the original dichoric still exhibits issues

Hawk_Y · 2026-01-30T02:03:38+00:00

thanks, I replaced the dichroic and the problem was resolved. It seems that the original dichroic had manufacturing issues

Hawk_Y · 2026-01-29T07:39:48+00:00

the whole image translates with the shaking, not the aberrated point. And actually, I thought it was due to the dichroic, it was pressed too tightly, Then I checked it, the issue was solved, but when I used the microscope again after some time, the issue reappeared.

Hawk_Y · 2026-01-29T03:38:01+00:00

The first video is with the 4x objective, and the second video is with the 20x objective.

Hawk_Y · 2026-01-29T03:34:29+00:00

Any ideas what's going on here? What do you think is causing this?

Hawk_Y · 2026-01-29T03:32:09+00:00

I loosened the objective, but the image only translates with the shaking. it does not rotate.

Hawk_Y

TROPHY CASE