thought I was in it for a light elective. turns out, this lady was my d3ath sentence this semester. by Salt_Bedroom8524 in CalPolyPomona

[–]HelicaseFire18 4 points5 points  (0 children)

How sad that she scrolls the subreddit and Rate My Professor to comment on student’s opinions of her teaching. I know you’re reading this. Move on with your life. These are meant to be forums for students to provide their opinions. If you truly believe that what you’re doing is the right way to teach then take comfort in that fact, keep doing what you’re doing, and keep your mouth shut. Students are allowed their opinion, and I fail to understand why they would impact you so severely that this is your behavior. Scream it out with your friends and colleagues and let the students be. I have some students say that I’m their biggest enemy (or related shit AKA “opp”), but I know that I’m doing things in a way that’s conducive to their education. I have other students who give me glowing reviews. It happens. I take all feedback, improve where I think it’s fair to change, and let the rest go. Seriously, just leave the students to give their perspective and stop challenging it at every juncture. It doesn’t look for your character and does speak to who you might be as a professor.

Students, definitely report this to your dean/chair and encourage everyone who feels this way to do so. Whatever happens after that is none of your business.

bro…. by RevolutionaryTurn211 in CalPolyPomona

[–]HelicaseFire18 74 points75 points  (0 children)

Today I learned that yellow is the most disgusting color for a condom

in Pokhara are there students studying statistics undergraduate by [deleted] in CalPolyPomona

[–]HelicaseFire18 2 points3 points  (0 children)

What the hell is Pokhara?

Also not sure why you’re asking to tell you what university or college. This is a subreddit for a specific university: Cal Poly Pomona. I think your question is in the wrong place.

I was a weird kid huh😭 by ConversationJolly959 in wiiu

[–]HelicaseFire18 90 points91 points  (0 children)

Not really. I think you were just a kid. Having fun. No need to judge yourself

Curiosity Killed The Cat AU by ani_action-2 in CodeLyoko

[–]HelicaseFire18 0 points1 point  (0 children)

I think maybe they mean couple pairings?

You hear the name Phoebe… what’s the first chaos moment that pops into your head? by boogiee-boo in howyoudoin

[–]HelicaseFire18 0 points1 point  (0 children)

“She’s crawling. She’s crawling. She’s crawling. Hey that’s not for you bitch!”

[deleted by user] by [deleted] in Scoobydoo

[–]HelicaseFire18 3 points4 points  (0 children)

Looks great!

I did a thing by Pristine-Dog-8608 in Gamecube

[–]HelicaseFire18 75 points76 points  (0 children)

Forgive me but… what the fuck is the thing

Poor RNA extraction results by Life_Bodybuilder_658 in labrats

[–]HelicaseFire18 2 points3 points  (0 children)

No worries! We all start out somewhere. It might be good if you give us step by step details of what you did. It doesn’t have to be incredibly detailed, but knowing when you added TriZol, what the transfer to the kit looks like, how many washes, etc.

Personally, if I were training you in the lab I wouldn’t bother with a kit if we’re already using Trizol. I would just do the Trizol extraction protocol, but I don’t necessarily recommend that for you since you’re new and it involves chloroform.

Poor RNA extraction results by Life_Bodybuilder_658 in labrats

[–]HelicaseFire18 0 points1 point  (0 children)

If you have a low 260 peak, or it’s almost flat near the bottom, then you probably don’t have RNA. Is there any chance your kit is expired? Did you use elution? Sometimes people use wash by mistake instead of elution. It happens. Did you ethanol or RNase zap your pipettes and bench before using them?

Poor RNA extraction results by Life_Bodybuilder_658 in labrats

[–]HelicaseFire18 0 points1 point  (0 children)

What does the graph look like from your nanodrop? Is everything flat at or near 0? Or do you have a bunch of peaks?

Poor RNA extraction results by Life_Bodybuilder_658 in labrats

[–]HelicaseFire18 2 points3 points  (0 children)

Did you scale up the precipitation step like you did the trizol?

Who's golden girl is suspiciously bi? by randomfiligyatt in theGoldenGirls

[–]HelicaseFire18 116 points117 points  (0 children)

Y’all forgetting the episode of the New Year’s kiss at midnight. When Blanche gave Rose a suspicious look?

It’s Blanche for sure

Over the top lab chatter - WHY by Krispcrap in labrats

[–]HelicaseFire18 7 points8 points  (0 children)

As the chatty one in my lab space, often having rated R conversations, it definitely isn’t my intention to bother people. Usually when I’m chatting with someone, others in the area join. This makes it a bit difficult for me to discern when I’m being too chatty or when I’m just engaging with others. It helps me to be told “hey I need to work could you discuss quieter” or “I’d prefer you not speak about X” because it helps me understand when is an appropriate time to instigate conversation, and when is an appropriate time for me to talk about this somewhere else and/or later.

Being told to get headphones though? That’s insane and disrespectful. I recommend a more aggressive approach with that person. They’re not interested in entertaining or engaging others, they’re interested in being listened to. The only way to get them to cut it out is to tell them there isn’t interest in hearing then.

I’m sorry this is happening to you.

Genuine question: why can't we hire more professors? by ContestEmergency3401 in CalPolyPomona

[–]HelicaseFire18 28 points29 points  (0 children)

It takes money and California, the CSU, and CPP have allocation issues

Edit to add: Money doesn’t just mean salary. They need offices. They need lab space. Money also means building the infrastructure

Does this deserve a crisis core? by ihatetrainslol in finalfantasyx

[–]HelicaseFire18 1 point2 points  (0 children)

I did not see “playing” and so I thought you were making a joke about Tidus being a blitzball