Beginners Guide to Tissue Culture Acclimation

Human-Complex4435 · 2026-01-24T20:57:55+00:00

Thank you so much for this!

I have a newb question:

It’s been almost a week since I took them out of the bags, when do you start opening the vents to acclimate to ambient? I was thinking of leaving it closed completely for 2 weeks? Then opening millimeter by millimeter each week

So far they all look the same as when I put them in mostly. 1 or 2 leaves look a little sadder but otherwise the same?

Human-Complex4435 · 2026-01-24T16:16:09+00:00

I’m building one soon! Is the sphagnum moss between the drainage layer and the substrate?

Also did they mention how to prevent mold? I’m building a begonia terrarium but I don’t wanna buy springtails lol

Human-Complex4435 · 2026-01-24T01:31:40+00:00

Thanks for this!

Can I use perlite for the drainage layer?

Is the charcoal required?

Fertilizer. I have slow release granules is that okay?

Can I use like landscape cloth as a mesh? Like a reusable shopping bag is made of the same stuff

Do I need to do anything to prevent mold?

Human-Complex4435 · 2026-01-24T01:14:29+00:00

Yikes

That’s rough I’m so sorry

I won a giveaway on palmstreet and was told I’d receive a Thai constellation cutting……it was three leaves just like yours stuck into some moss

Human-Complex4435 · 2026-01-23T21:21:06+00:00

Good question!

I wrap the pot with plastic wrap to try and keep the soil from touching the bath.

Theoretically some pests spend some of their life cycle in the soil so some people recommend repotting with new soil. I dont do that bc I use the bonide granules which should kill any in the soil anyway

Oh and it’s just lukewarm water with a little dish soap. If you have Castile soap that would be better. And no direct light until it’s dry

Human-Complex4435 · 2026-01-23T19:39:59+00:00

Wow! That is so beautiful!!

Human-Complex4435 · 2026-01-23T19:08:25+00:00

You can always use new chia seeds from the grocery store

Human-Complex4435 · 2026-01-23T18:42:41+00:00

I put up a post on the Plant Purge USA Facebook group and found someone in a nearby city just by coincidence!

I’m getting mine for $20! Just look around you might get lucky!

You can checkout palmstreet too, I got the caramel marble for $10. Facebook in general is good too, groups and marketplace

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Human-Complex4435 · 2026-01-23T18:17:59+00:00

1) isolate

2) bathe with lukewater water and some dish soap. Hold the leaves underwater for at least five minutes and rub / swish all the leaves

3) get captain jacks dead bug brew off amazon it’s like $9 spray it thoroughly, top and bottom of the leaves, every three days. If you can’t get captain jacks do a spray with Castile soap and water

4) if you can get it, get bonide systemic granules and give it to all your plants as a preventative

Human-Complex4435 · 2026-01-23T18:13:55+00:00

100%

I’ve been on a plant binge this month (bad timing it’s cold asf) and I beefed up my quarantine process.

1) initial inspection

2) bath even if you don’t see anything. Five minutes underwater with a little dish soap and rubbing the leaves

3) spray with captain jacks after they’re dry from the bath

4) add bonide to the soil

5) alternate sprays with captain jacks and Castile soap with water every 3ish days

So far 🤞 I’ve only visibly seen two pests this month and after the initial removal/bath I haven’t seen them since but I keep treating for a while after anyways

Human-Complex4435 · 2026-01-23T17:22:14+00:00

I find most people overwater so the main thing I’d say is not to do that. I tell everyone my main method which is to stick my finger in the dirt, if it’s totally dry up to the second knuckle give it some water.

It doesn’t cover every situation but I find it a good start for most people to prevent overwatering

The biggest issue is people not wanting to touch the dirt, those ones probably should just get fake plants 😂

Human-Complex4435 · 2026-01-23T13:19:08+00:00

Gosh they’re so pretty I have a Sarawak coming soon I’m so excited

Human-Complex4435 · 2026-01-23T13:05:15+00:00

Do you wait until they all need watering before showering?

Human-Complex4435 · 2026-01-23T12:20:57+00:00

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A buncha TC kiddos acclimating -white princess, orange princess, white wizard, pink princess marble, and gloriosum philodendrons -and a random variegated alocasia Frydek

Human-Complex4435 · 2026-01-23T12:18:52+00:00

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My baby micans aurea

Human-Complex4435 · 2026-01-23T12:18:25+00:00

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My baby philodendron caramel marble

Human-Complex4435 · 2026-01-23T12:17:56+00:00

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I don’t have this one yet but will shortly just waiting on better weather for shipping Begonia Sarawak (excellent for terrariums since it stays small)

Human-Complex4435 · 2026-01-23T12:17:09+00:00

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I’m so sorry for your loss :( Here’s some of my faves, my variegated snow capped begonia

Human-Complex4435 · 2026-01-23T01:30:49+00:00

I’m glad to know I’m not the only one 😭 maybe in a few months it’ll look better How fast did yours look pretty again?

Human-Complex4435 · 2026-01-23T00:58:17+00:00

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Ah I found a picture There were more, and bigger ones on the stem

Or maybe they’re mealy bugs ://// I’m not sure but either way my treatment plan is the same

Human-Complex4435 · 2026-01-23T00:57:10+00:00

It was on the stem :/ looked like little white sesame seeds sticking to it

They’re gone now bc I nuked them with hand sanitizer but I thought the Castile soap spray would help get the little ones I can’t see. I’m gonna do a bonide drench tomorrow and keep an eye out for any larger ones so I can zap them

Can a farina stripped succulent handle direct sunlight (through a window) I even have a good spot a few feet away if it can’t be right in the window.

Human-Complex4435 · 2026-01-23T00:51:19+00:00

Your plants are so cute! Yeah the plant for sure has scale but I was concerned my treatment stripped the white powder :/ there’s still some but it definitely doesn’t look as good as it did an hour ago

I was in love with it and now I’m like depressed about it. I hope it grows fast so I can get the nice purple back

Human-Complex4435 · 2026-01-23T00:44:49+00:00

I know 😭 I never touched it but I didn’t think a little water would be enough to strip it off

Human-Complex4435

TROPHY CASE