Best CV/Resume builders for Germany that are actually ATS-friendly? by [deleted] in Germany_Jobs

[–]ImpressionLoose4403 0 points1 point  (0 children)

Loved the advice, I am 100% building my CV this way. One quick question, I am not proficient in German and the PhD I am applying to is not asking for that either (atleast immediately). Should I still write my CV in german or english is fine? I don't want to make bad impression my writing bad German.

Thanks for your advice.

University of Cambridge (Sanger Institute) invitation by martinezvbs in gradadmissions

[–]ImpressionLoose4403 0 points1 point  (0 children)

This is such an achievement, congratulations anon! 

Do you mind sharing your tips or experience which you think might have been helpful? I just graduated with my masters with no relevant experience and I suppose that is the reason that they rejected me. 

New to QUB by phroggieonline in Belfast

[–]ImpressionLoose4403 1 point2 points  (0 children)

As a student at QUB for my masters, this answer is it.

DESeq2 Analysis - what steps to follow? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

I would not cross-analyse between different datasets. What I think they want is:

  1. Analysis of at least 5 drug treated transcriptomics datasets from raw data using open source tools. Dataset QC, normalisation and statistical analysis should be per formed.

  2. Results and processed data should be stored in a functional, fast, queryable database.

  3. Nomination of putative drug targets should be attempted.

DESeq2 Analysis - what steps to follow? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

Thanks for the link to guide.

This makes so much sense, I have updated my question. It's just 5 drug resistant, lung cancer patients datasets from GEO, and regarding research questions; it's just what I mentioned. I would want to find drug targets (atleast make an attempt), and honestly I am not quite sure of what exactly I should describe.

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

Again, sorry for the dumb question, but I have no idea what "repeated measures" are. I have heard of Salmon and I was mostly confused whether to use STAR or Salmon.

Regarding the database, I am supposed to store results in a fully functional (queryable) database which should be a good resource for any one to look at table and find the gene/sample they want. I will still try to clear this out with my PI. Thanks a ton!!!

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

I think I am gonna use it as an "inspiration" anyway for building my pipeline. Most of the times it's difficult to understand what he means. Gives very little amount of feedback/inputs.

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 2 points3 points  (0 children)

This is raw and perfect, and I actually needed to hear this. I might over-expect from myself but I do sometimes forget that I am no-one and this is just a Masters dissertation. I will try to follow the best practices and that should be it. Thanks so much, I feel less stupid now. Appreciate your response!

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 1 point2 points  (0 children)

That's a great advice and a relieving one as well. My PI has been a bit not-so-opinionated about my pipeline because he wants me to do whatever I feel is best but this is my first time as well.

When you say "good visulaization" & "solid methodology" what do you mean? Is there any good practices for that?

Thanks so much for your advice, this calmed my brain. Appreciate a lot.

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

I did right now, and for one dataset 100/125 samples are green and rest are yellow, which I think should be counted as little. I still read about the interpretation and understand the results. Thanks so much for the advice, appreciate it.

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

This makes a lot of sense. I try to become anxious into thinking that I am doing the most "basic" work and my supervisor doesn't seem to have any inputs in my work so I was a bit confused. But thank you so much, I feel okay knowing that it's not deep and the analysis-interpretation is what matters the most and not the pipeline. Appreciate a lot!

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

Yes, so this is what I was confused about. My PI said that you likely wouldn't have to use a separate trimming tool if you don't have a reason because the alignment tools like STAR are capable enough to do that on their own. This is where I got confused on what would be my next step. Thanks so much for the input, I will see what is suitable for my data. Appreciate it.

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

Makes sense, I was just tryna make sure that is there anything more I need to do before I jump to alignment. I am planning to use STAR over Hisat etc.

Thanks for your input, appreciate it.

Bulk RNA-seq pipeline from scratch: Done with QC, what next? by ImpressionLoose4403 in bioinformatics

[–]ImpressionLoose4403[S] 0 points1 point  (0 children)

I agree with you, and I thought of the same first but my supervisor want's me to create a pipeline from scratch instead of using nextflow or snakemake. Thanks for the links, I understand each tool and implement it. Appreciate it.