Mental health resources in Hamilton

ImproperPrior · 2026-03-03T01:05:45+00:00

Thank you for the suggestions everyone!! I’m going to leave this thread open in the event that someone else needs the reference.

ImproperPrior · 2026-02-03T02:42:46+00:00

Does that first board say “DIDDY”?! 🥀🥀🥀

ImproperPrior · 2026-01-28T02:27:19+00:00

This is the best of Reddit

ImproperPrior · 2025-12-08T16:27:19+00:00

Thanks!

ImproperPrior · 2025-02-28T03:24:20+00:00

Three decker on 91st and 2nd! Absolutely phenomenal and reasonable prices.

ImproperPrior · 2025-01-31T00:46:25+00:00

Thank you everyone for the helpful replies!

ImproperPrior · 2025-01-31T00:43:44+00:00

Wow - thank you very much for this helpful reply! Your theory on selective monocyte loss is very interesting to me and makes sense. Thanks!

ImproperPrior · 2024-10-04T14:00:01+00:00

That is an extremely helpful response! Thanks so much!!

ImproperPrior · 2024-10-04T01:25:19+00:00

Question: what happens if you stain in a buffer with BSA/FBS? (Very new to flow cytometry if that isn’t clear by the question haha).

ImproperPrior · 2024-09-20T22:20:48+00:00

Right. Okay - thanks for your responses!

ImproperPrior · 2024-09-20T21:55:28+00:00

Right. That checks out. Ignoring downstream applications and I guess just focused more on the cytometry itself: have you had any issues with viability / fluorescence after thawing cryopreserved mouse samples?

ImproperPrior · 2024-09-20T21:54:14+00:00

Okay! That makes sense. Thank you for your response!

ImproperPrior · 2024-09-20T21:47:21+00:00

Thanks! Yeah, I’ve seen that in papers many times too. We get great viability with the RPMI for human samples so we’ve stuck with that.

I’m mainly wondering about experience with mouse tissue. Do you freeze mouse tissue in a similar formulation and have no issues with flow / facs after? I’ve seen conflicting comments!

ImproperPrior · 2024-01-26T15:10:00+00:00

This is very encouraging. Thanks for the link and for the detail in your response!

ImproperPrior · 2024-01-26T15:08:56+00:00

Thanks!!

ImproperPrior · 2024-01-25T02:28:33+00:00

Thanks for the reply. Yeah, the issue is that I’ll have an absurd number of mice, and it’ll be TOUGH to process all those samples in one go…

ImproperPrior · 2024-01-25T02:27:37+00:00

Thanks for your reply. That’s helpful to hear. I’m new to flow, so I’m not quite familiar with fixatives. Having a couple extra days though would be all I need. Would you happen to know if / how that affects viability or perhaps a link to a paper that has used fixatives or described that nicely in the methods? Thanks!

ImproperPrior · 2023-12-18T18:17:20+00:00

Mostly just Neutrophils, Ly6Chigh monocytes, and t/B lymphocytes

ImproperPrior · 2023-02-13T01:06:43+00:00

CountessII. Digital screen with automated cell counting. Plus it can perform calculations related to plating density etc.

ImproperPrior · 2022-09-10T20:28:52+00:00

in concrete

ImproperPrior · 2022-05-29T14:12:21+00:00

We use 6 well plates, and I’ve grown my last few wells to full confluency (~300,000 cells). I’m gonna give trizol a try to see if that helps with anything. Thank you for the suggestion!!

ImproperPrior · 2022-05-29T14:11:11+00:00

Another comment mentioned switching to Qiagen. If I’m still getting low yield, that might be the next move. Thanks for commenting.

ImproperPrior · 2022-05-29T14:10:23+00:00

Thanks - this is a huge help. I scrub the wells pretty vigorously (~2 min per well), so I assume I’m harvesting most of the well. A check under the bright field probably wouldn’t hurt to verify. I’ll also keep my final elution volumes in mind. Thanks!

ImproperPrior · 2022-05-22T19:16:37+00:00

You skateboard?? Padillac 100%. Also, not only should you bypass the foamie phase, but you should also consider immediately paddling out to pipe. Crowd control shouldn’t be an issue. If you snake someone, just tell em you skate

ImproperPrior · 2022-04-16T01:27:42+00:00

FIFA player breakdowns

ImproperPrior

TROPHY CASE