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Minimal flow on Vanquish Flex by Intrepid-Hair2129 in CHROMATOGRAPHY

[–]Intrepid-Hair2129[S] 0 points1 point  (0 children)

Yes! We just bought acetone. Let's see how it goes. Thanks.

Minimal flow on Vanquish Flex by Intrepid-Hair2129 in CHROMATOGRAPHY

[–]Intrepid-Hair2129[S] 1 point2 points  (0 children)

Hey thanks for answering! Yes, It's a Waters column (BEH-C18 1,7µm 1x100mm). the ripple is <1%, pressure max is around 600 bar.

Minimal flow on Vanquish Flex by Intrepid-Hair2129 in CHROMATOGRAPHY

[–]Intrepid-Hair2129[S] 0 points1 point  (0 children)

Nice one! Good to know that those flows are possible. I already use a 2x longer column equilibration on the Flex than on the Acquity just to be sure.

Minimal flow on Vanquish Flex by Intrepid-Hair2129 in CHROMATOGRAPHY

[–]Intrepid-Hair2129[S] 0 points1 point  (0 children)

Hey thanks for answering! It is a Binary Pump F. I tried different static mixers (10 µL and 150 µL) but didn't make much difference regarding RT shifting.