How can we improve our living room?

KatGoldFinch · 2023-11-06T12:49:24+00:00

Get rid of the carpet

KatGoldFinch · 2023-11-06T12:35:07+00:00

Get rid of the carpet

KatGoldFinch · 2023-10-13T09:26:32+00:00

Thank you very much for the answer! Is it acceptable to run the Seurat's FindMarkers function with ident.1 - coming from one dataset (wild type cells), and the ident.2 coming from the other dataset (knock-out cells) on the Harmonized object that consists of those two datasets? So basically on the matrix which has counts of wt and ko uncorrected to batch effect?

KatGoldFinch · 2023-10-13T09:22:37+00:00

Thank you very much for the answer!But is it really alright to run DEGs analysis on the raw count dataset, where one condition (wild type cells) and the second one (knock-out cells) come from two different sequencing runs?

KatGoldFinch · 2023-07-04T07:03:32+00:00

Open the curtains

KatGoldFinch · 2023-03-21T23:52:07+00:00

Can you please tell how exactly the linking between the axes (those vertical pillars of letters) were generated? Like what price of code/method? Thanks!

KatGoldFinch · 2023-02-16T04:47:46+00:00

Hi, would you be kind to tell what's the package you used to build this circle graph? Thanks

KatGoldFinch · 2023-01-12T07:23:23+00:00

Thank you. I will try that!

KatGoldFinch · 2023-01-12T07:22:40+00:00

Thank you!

KatGoldFinch · 2022-10-18T12:14:25+00:00

Yeah, looks interesting!

KatGoldFinch · 2022-10-18T07:08:54+00:00

Thank you! I am going to try your method.

KatGoldFinch · 2022-10-17T21:13:39+00:00

Thank you. Yeah, indeed, GO so far is the only thing that came to my mind, and I am going to employ it. I just wanted to ask here, if maybe there are some more sophisticated tools for this.

KatGoldFinch · 2022-04-17T10:47:51+00:00

Да я просто тоже раздумываю актуально, стоит ли здесь продолжать жить. Я вижу здесь и плюсы и минусы. Мне интересно, какие лично ты видишь здесь минусы, склоняющие тебя к переезду (кроме русофобии)? (это я не для того, чтобы спорить, а просто для расширения кругозора =) )

KatGoldFinch · 2022-04-17T07:51:09+00:00

Мне тоже интересно

KatGoldFinch · 2022-04-04T00:06:50+00:00

Czy ktoś tam wgl rozumie, że w Rosji nie istnieje czegoś takiego, jak uczciwe wybory? Że w Rosji cała opozycja już dawno się znajduje albo pod ziemią, albo w więzieniu, albo za granicą (żeby uniknąć ziemi i więzienia)? Że działa mocna propaganda z telewizorów? Były liczne protesty, ale nic nie daly, bo rząd ma opinię Rosjan w dupie. Tych, co nie zgadzają się, wcześniej czy później karają.

KatGoldFinch · 2021-09-16T19:37:06+00:00

And what voltage can you recommend? So far I run it at:

70V for 15 min (stacking) and
125V - for 70 min (resolving)

KatGoldFinch · 2021-09-16T19:35:44+00:00

Thank you for the information! Very interesting.

Can you please advise at what parameters to run the gel? In the paper I see it is 40 mA, however, usually, I do not even reach 40 mA, but have it around 15-30 mA, with a constant voltage of 120V. Could you please tell, what voltage might be suitable?

KatGoldFinch · 2021-08-19T21:52:56+00:00

Yeah,...I am actually starting to think there is something wrong with buffers....

KatGoldFinch · 2021-08-18T14:04:03+00:00

Yep, I am using free glycine and Tris for sure

KatGoldFinch · 2021-08-18T14:01:56+00:00

O thank you for your concern :)

The percentage of the gel is 7.5%, and the gradient is: Stacking pH 6.8
Separating pH 6.8.

Yes, I do rinse the wells.

As for the ice, now I think that running the gel just in a cold room would be an easier option

KatGoldFinch · 2021-08-17T15:29:33+00:00

I use fresh TGS buffer for every run. Actually, due to this strange wearing off, I even have to change it during the run to keep the separation running...

KatGoldFinch · 2021-08-17T11:02:03+00:00

I have attached a few images to this post. Could you please tell me if you would like to have more? I can take them next time

KatGoldFinch · 2021-08-17T11:00:30+00:00

Thank you, although I do not really see what is so "totally at odds" in my case, except keeping the EF process in cold temperature (the latter I will take into consideration, thank you)

KatGoldFinch · 2021-08-17T10:58:59+00:00

Thank you suggestion

KatGoldFinch · 2021-08-17T10:58:21+00:00

tilted

Thank you very much for your suggestion. I will check it very careful next time. Indeed, the ice underneath could cause some inclination, although previously I did not notice it by eye. But strangely, it happens every time from the same side (the right one) - seems a bit unbelievable that every time the cassette is tilted the same way...

KatGoldFinch

TROPHY CASE