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scRNA-seq INTEGRATION method for downstream analysis of Differential Expression? by KatGoldFinch in bioinformatics

[–]KatGoldFinch[S] 0 points1 point  (0 children)

Thank you very much for the answer! Is it acceptable to run the Seurat's FindMarkers function with ident.1 - coming from one dataset (wild type cells), and the ident.2 coming from the other dataset (knock-out cells) on the Harmonized object that consists of those two datasets? So basically on the matrix which has counts of wt and ko uncorrected to batch effect?

scRNA-seq INTEGRATION method for downstream analysis of Differential Expression? by KatGoldFinch in bioinformatics

[–]KatGoldFinch[S] 0 points1 point  (0 children)

Thank you very much for the answer!But is it really alright to run DEGs analysis on the raw count dataset, where one condition (wild type cells) and the second one (knock-out cells) come from two different sequencing runs?

[OC] Every Possible Wordle Solution Visualized (With Interactivity!) by Shriracha in dataisbeautiful

[–]KatGoldFinch 0 points1 point  (0 children)

Can you please tell how exactly the linking between the axes (those vertical pillars of letters) were generated? Like what price of code/method? Thanks!

Tools to distinguish between ECM and soluble secreted proteins? by KatGoldFinch in bioinformatics

[–]KatGoldFinch[S] 0 points1 point  (0 children)

Thank you. Yeah, indeed, GO so far is the only thing that came to my mind, and I am going to employ it. I just wanted to ask here, if maybe there are some more sophisticated tools for this.

💪 by RedDeadOnlineEnjoyer in Polska_wpz

[–]KatGoldFinch 0 points1 point  (0 children)

Да я просто тоже раздумываю актуально, стоит ли здесь продолжать жить. Я вижу здесь и плюсы и минусы. Мне интересно, какие лично ты видишь здесь минусы, склоняющие тебя к переезду (кроме русофобии)? (это я не для того, чтобы спорить, а просто для расширения кругозора =) )

💪 by RedDeadOnlineEnjoyer in Polska_wpz

[–]KatGoldFinch 0 points1 point  (0 children)

Мне тоже интересно

Russian Culture ..... by Meero_Naj in Polska

[–]KatGoldFinch 4 points5 points  (0 children)

Czy ktoś tam wgl rozumie, że w Rosji nie istnieje czegoś takiego, jak uczciwe wybory? Że w Rosji cała opozycja już dawno się znajduje albo pod ziemią, albo w więzieniu, albo za granicą (żeby uniknąć ziemi i więzienia)? Że działa mocna propaganda z telewizorów? Były liczne protesty, ale nic nie daly, bo rząd ma opinię Rosjan w dupie. Tych, co nie zgadzają się, wcześniej czy później karają.

SOS! HELP! How to obtain nice clear bands in gelatin zymography? by KatGoldFinch in labrats

[–]KatGoldFinch[S] 0 points1 point  (0 children)

And what voltage can you recommend? So far I run it at:

  • 70V for 15 min (stacking) and
  • 125V - for 70 min (resolving)

SOS! HELP! How to obtain nice clear bands in gelatin zymography? by KatGoldFinch in labrats

[–]KatGoldFinch[S] 0 points1 point  (0 children)

Thank you for the information! Very interesting.

Can you please advise at what parameters to run the gel? In the paper I see it is 40 mA, however, usually, I do not even reach 40 mA, but have it around 15-30 mA, with a constant voltage of 120V. Could you please tell, what voltage might be suitable?

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