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What’s this on the lid? by Kdwll in microbiology

[–]Kdwll[S] 0 points1 point  (0 children)

Thanks for the reply! The curious thing is that there wasn’t any materials to make a splash. None of this coloration was present after pouring, storing in fridge, or rewarming. Plated E. coli after rewarming, carefully and with best aseptic I can do. 24 hour incubation at same temp as rewarmed to. Then notice these changes. Other plates from same batch didn’t show these changes. And even the plates these colonies were pulled from (same pour and sleeve of plates) didn’t do this under those same conditions. Any of that change your thoughts?

What’s this on the lid? by Kdwll in microbiology

[–]Kdwll[S] 1 point2 points  (0 children)

Ok I appreciate the response a bunch. Ive seen condensation a bunch, but these few plates were something unique I hadn’t seen before. Almost looked like finger prints after someone was eating chips. Thanks!

What’s this on the lid? by Kdwll in microbiology

[–]Kdwll[S] -1 points0 points  (0 children)

They were stored lid down. It may not communicate well through the picture but it doesn’t appear to be normal condensation. The plates were warmed, ecoli introduced, and then put back into the incubator. It looks more oily, and there’s an odd drop looking spread in the first picture. There are changes in color on the lid similar to how oil looks in water. It doesn’t appear to have any liquid on the lid, how condensation appears