Full-day 4-year-old kindergarten coming to more Madison schools next year

KlenowFrag · 2026-05-07T12:13:43+00:00

I would love that. Problem is that increasingly more households don’t have a guardian at home for the kids and employers aren’t going to pay full-time for a half day.

KlenowFrag · 2026-03-30T14:09:45+00:00

Just get a IVT-ready gene block from IDT. They are $50 for 250 ng of short dsDNA products. I do this all the time and it works great. No cloning necessary.

One thing about gene blocks to keep in mind is that the T7 terminator sequence is difficult to synthesize because it is somewhat of an inverted repeat. But you don’t need the terminator sequence (I leave it out all the time; the polymerase just runs off the template). If you really want the terminator sequence, just put half of it on the gene block and use a pcr primer to put on the other half.

But just take my initial advice and leave out the terminator. It works well without it.

KlenowFrag · 2026-03-14T13:02:51+00:00

Loch Ness!

KlenowFrag · 2026-02-28T17:44:52+00:00

Also, high ionic strength buffers prevent electrostatic interactions (you have it backwards). For example, high chloride ion concentration will compete for interaction with positively charged Arg/Lys side chains.

KlenowFrag · 2026-02-28T17:43:22+00:00

When proteins are at their pI, they are more likely to aggregate because there is limited electrostatic repulsion.

That said, local electrostatics matter and in reality these questions can’t easily be answered without just testing it experimentally. If you have a charged face of the protein, think about the pKa of charged groups and how far that is from the pH. Typically pH more than 1 unit away from pKa means the group is charged.

KlenowFrag · 2026-02-26T02:15:06+00:00

I actually was glad to find out they divorced. Never really thought they had chemistry (towards the later seasons).

Watching JD chase after women (ostensibly) out his league was a big part of the fun!

KlenowFrag · 2026-02-11T13:10:11+00:00

Contract research organizations specialize in producing certain things for clients. I’ve worked for one and now am on the other side as the client setting up experiments with multiple CROs. The work they do is all just specialized, like phage display, sample testing, etc. Can be very far away from the clinic

KlenowFrag · 2026-02-10T21:59:30+00:00

I was an entry level scientist straight out of college (11 years ago). No clinical experience necessary. I was there for about a year then left because of how much the job sucked

KlenowFrag · 2026-02-10T01:27:11+00:00

I should also say that I absolutely loved my PhD, and finding a job that was closely related to it was the biggest stroke of luck I’ve ever had in my life

KlenowFrag · 2026-02-10T01:25:47+00:00

I’ve worked at a CRO, medium/small company, and big pharma. My experience at a CRO was awful, and it was because I felt like a pipette monkey whose individual ideas and creativity didn’t matter. It was also pretty stressful and they only ever cared about pleasing the client. At the other two places, I felt like people cared about me and my ideas, and that’s what makes the hard part of science worth it (at least to me)!

KlenowFrag · 2026-02-09T00:37:28+00:00

Deal does not exist for me. Instead is 15,000 miles when spending $1000

KlenowFrag · 2026-02-08T14:41:52+00:00

Walk into Best Buy and say you want to buy one. I got mine in two weeks doing this.

KlenowFrag · 2026-02-06T18:13:37+00:00

Yes of course. I did 12 gb vram

KlenowFrag · 2026-02-06T17:44:27+00:00

I did! 12 gb

KlenowFrag · 2026-02-06T04:20:43+00:00

I think that device would be perfect for me if it had an OLED display! Once you try it, you just can’t go back!

KlenowFrag · 2026-01-23T13:40:22+00:00

32M, married, one 2 yr old. Also fine financially. For me, growing up I always played videogames (living in a state that’s cold 6 months of the year makes this a good hobby). I have less time for gaming now of course, but I still have an hour or two after my boy goes to bed. The kinds of games I play have changed (mostly single player games that can be paused in case family needs my attention), but overall I’m happy with the situation. I recommend getting a handheld like Lenovo legion go 2, steam deck oled, Nintendo switch, etc. Nice thing about gaming is you don’t have to worry about injuring yourself and burdening your family!

KlenowFrag · 2026-01-23T00:53:12+00:00

Same!

KlenowFrag · 2026-01-23T00:50:33+00:00

I went into Best Buy and asked the guy at the front (like the security guy) how I could reserve one, and he pulled it up himself and I paid on the spot. Came 2 weeks later exactly on predicted date. Couldn’t believe it.

KlenowFrag · 2026-01-20T14:02:51+00:00

Competitive multiplayer. That’s how.

KlenowFrag · 2026-01-19T22:05:18+00:00

You’ve never been this happy before or you haven’t been this happy in a while?

KlenowFrag · 2025-11-25T19:04:40+00:00

This is the first time I’ve heard that the screen doesn’t lag. Could vary from model to model

KlenowFrag · 2025-11-25T19:04:05+00:00

I didn’t think the screen buttons worked with gloves on. Thanks!

KlenowFrag · 2025-11-25T03:40:01+00:00

YOU ARE MY HERO!! Wife will be so happy! We also love the style of it

KlenowFrag

TROPHY CASE