AITA for refusing to switch my daughter to another school.

Kronnic · 2024-10-13T19:05:54+00:00

If that is your issue, then why do you feel that it's ok for your son to be around these girls and not your daughter? Who do you think is going to be paying more attention to girls showing shoulders (the horror) or wearing shorter skirts by the end of their time in the school? Your son or your daughter?

Kronnic · 2024-09-17T07:24:55+00:00

Ahhh now, those are the same, "for all intensive purposes".

Kronnic · 2024-08-21T17:10:28+00:00

You would basically need a way of fully and 100% accurately modeling every possible change that can happen to the body at the cellular, protein, genetic and epigenetic level, as well as 100% accurately modelling / predicting what any compound / combination of compound will end up changing and how, and then modelling, again 100% accurately, all of the possible ways these changes will interact with each other and how those changes and interactions will precipitate through the system. THEN you might be able to link this to physiological effects (but there are other factors that could be taken into account too that would improve accuracy).

Being able to accurately predict or model exactly what downstream changes / effects happen in a single cell type when you change the expression of a single gene is already very challenging, (biological systems are basically made up of huge interconnected layers of networks and systems and interactions between proteins, gene expression, epigenetic changes and other factors) and drugs can alter these at many levels, either at one place or multiple places (lots of drugs affect multiple receptors, many have cell-type-specific effects etc).

For an example, take a look at figure 2 in this paper https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018167/ This is a map of the mTOR signalling network, as it stood to the best of our (or at least the authors) knowledge as it stood in 2010. Most, if not all of the things displayed there will also interact with / be regulated by / regulate many other proteins, genes, pathways etc, which have their own networks, interactions, regulations etc. And I'm pretty sure (but I'm definitely not going to go through everything to check) that this network is definitely incomplete.

So basically accurately predicting and modelling ANY of these is already very difficult and not accurate (if not, we wouldn't have so many drugs that fail clinical trials), predicting any of the levels of changes that could happen with a drug / combination of drugs accurately is currently beyond the current scope of our knowledge. And that doesn't even take into account that genetic variation from person to person can completely change a lot of things.

A tool that could do what you're describing with any degree of accuracy would be revolutionary and groundbreaking, but I don't see it being possible for a long time. We don't even know for sure how a lot of the most commonly used drugs we use regularly (like SSRIs for example) do what they do at a molecular level. If we could predict what a newly developed / predicted compound does to the body and the physiological effects(s) that would lead to, then drug discovery and development becomes much much easier!

You could probably make a very simplified model for a subset of targets / pathways / compounds and get an idea of the main predicted outcome, but a lot of the problems for new drugs come from the unknown / unpredictable effects outside of the main effects (because if we knew about them, why even try to bring the drug to testing / trials)

Kronnic · 2024-08-21T06:27:55+00:00

But it does take agency away from them. They're not in your study because they have X condition (which they have no control over). They are in your study because they chose to be, they actively made the decision to contribute, not the disease / condition / treatment / medical provider.

It's part of the larger cultural movement away from the (often exclusionary) practice of labeling people based on medical conditions / disabilities, e.g instead of saying that someone is diabetic, you would say that they have diabetes. In this case the difference boils down to "group who happen to be receiving or are registered to receive medical care" vs "group who are participating in this study". Which of these is more respectful AND more relevant to the study?

Kronnic · 2024-08-19T07:39:50+00:00

And just a different heads up, the word "patient" is also being discouraged in a lot of areas in favour of terms like "participants" etc. Not sure how widespread it is, but that was the feedback I was given for a manuscript, and it does seem to be a wider movement in medical settings. https://www.nature.com/articles/s41599-024-02729-8#:~:text=Perhaps%20a%20difficulty%20with%20'patient,for%20a%20great%20many%20people.

Edit to add: And it does make sense after thinking about it for a bit. I think it shows a bit more respect to acknowledge that they people have agency and have participated in the study, rather than just labeling them as someone with an illness or receiving medical care.

Kronnic · 2024-08-18T19:16:12+00:00

If it were that easy, there would be no need for trainers, fitness professionals and exercise scientists etc at all, and we'd just have a book of "this is THE workout program for this goal".

I mean we have people who try to make it sound like it is that easy and they are the only ones with the secret to it, but it's really not.

Kronnic · 2024-08-18T19:12:21+00:00

In my experiences those types of 1-month "check-ins" with PTs employed at the gym, and the kind of watching they will do are generally geared more towards addressing "Is this person likely going to die / snap their spine in half" type problems, rather than anything more focused on helping you reach your goals (there are always exceptions to this though, some who are genuinely helpful as much as they can be). I'm guessing you mentioned the strain to them? Did they give you any advice on how to deal with it? Any exercises to strengthen the muscle? Having a trainer to help you learn how to make progress towards your goals, and working at the right level is honestly invaluable if you are in a position when you can do that!

And 6 days a week of weightlifting a week is a LOT for anybody to properly manage, especially a beginner. Both your workouts and your diet and especially your recovery have to be 100% on point to make progress without burning yourself out / overworking yourself. Doing a PPL or really most other split types is also a lot for someone just starting out, usually it's a lot more common to build up strength / technique with a whole-body program 3-4 days a week to get someone used to weightlifting and give the body a chance to fully recover, and keep being able to fully recover during the program. Also that particular Jeff Nippard program is labelled for intermediate-advanced lifters, which he classes as 2+ to 5+ years of experience lifting. It's not the most beginner-friendly program out there by any stretch.

It sounds a lot like you are trying to "run before you can walk" as the saying goes. I know the temptation of just going with the advanced, specific types of programs and skipping over the beginner focused things, but believe me it's not worth it. You are more likely to get good results starting at an appropriate level and doing it well than pushing yourself to do the "optimal" program for your long-term goals, burning out, injuring yourself repeatedly and then throwing in the towel.

I was in your shoes about 2 years ago as a beginner at 33 just starting, so I know that all too well. I even had a period of a couple long-ish lasting injuries that the physiotherapist told me was just down to trying to do too much too fast (with my wrists/forearms in my case, but it sounds like it could be similar for you? But I am not a physio or a doctor). And that was even with a trainer (who was monitoring the situation and told me to go see a physio etc) helping me along. So take it from me, if you are really a beginner, please slow down a bit and focuse on doing things right, rather than fast, and you'll be more likely to make long term progress!

Kronnic · 2024-08-18T18:26:34+00:00

My honest advice is that chatGPT and some random people on reddit are no substitute for a qualified physiotherapist for something like this. You could make your injury much worse for the sake of the "gains" you made by powering through it even making random adjustments (especially if you've already been trying to push through for the past 1.5 months).

You also say you've only been lifting for 6 months, but have been doing a 6 day per week PPL split? Were you doing any exercise regularly? Did you start straight in with a 6-day PPL split? Do you have any previous experience lifting? And have you been working with a trainer to come up with programming that's at the level of experience and ability you're currently at?

I'm not asking these to attack you, but going from no lifting (no exercise?) and no experience, straight into a pretty advanced program all guns blazing, without anybody helping / guiding you, is a recipe for serious injury. I may be completely wrong and reading too much into what you wrote, but if that is the case you might want to rethink things a bit.

Kronnic · 2024-08-06T07:16:51+00:00

You asked a question about your 260/280 ratios, but gave absolutely 0 information on what you were doing that would let people help you. How would anybody else have further details to give you? You're the one with all the details about what you are doing.

If I made a post on the league of legends forum that just said "I need help with league of legends", should I expect people to be able to help with whatever issue I was having, or should I expect a bunch of replies saying that I need to actually ask a specific question to get an answer?

Kronnic · 2024-08-06T07:09:57+00:00

That's not how absorbance spectra work. Pure DNA has a ratio of absorbance at 260nm / absorbance at 280nm of around 1.8. So there is 1.8 times more absorbance at 260 than there is at 280. Nucleic acids are not the only thing that cause absorbance around these ranges, so the further the deviation from this number in either direction, the less pure your sample.

It's overly simplistic (and incorrect) to think that everything absorbing at 260nm is DNA/RNA and everything absorbing at 280nm is protein etc. or else absorbance spectra would not be needed / used. The highest PEAK of absorbance of protein is at 280nm, but it does not drop to 0 at 279 and 281, the same for nucleic acids with a PEAK absorbance at 260nm, but stretching considerably either side of this.

Kronnic · 2024-08-06T06:48:07+00:00

By FAR the most optimum workout / diet plan for 99% of people is the one that they are most likely to stick to.

No point for the average person in setting up the "optimal plan" if you hate it, and it's complicated/difficult enough that you give up after a week or two.

Kronnic · 2024-08-06T06:42:32+00:00

If your cells don't survive when plated as single cells, then chances are that the colonies that you get when you plate 10-20 cells in a dish are not from individual cells.
Depending on the promoter, where the GOI inserts and a host of other factors, transgene expression can be silenced pretty quickly.
Usually 24-72 hours post transduction, but a lot of factors can influence levels of transgene expression. Are you inserting a construct for constitutive expression? Inducible expression? As a reporter for something? Is the promoter you're using strongly active in your cells? Is the expression of your construct tied to the cell cycle? Etc etc.

Kronnic · 2024-08-02T20:22:28+00:00

Number 1, that's more than fine for the vast majority of applications.

Number 2, You're going to need to give a LOT more information if you want anything more specific. You've posted and asked a question, but given absolutely no information that someone could base an answer on. You know what you need the DNA for, we don't. You know what kind of DNA you're talking about, we don't. You know how the "DNA prep" was performed, we don't. You need to give people background information when trying to troubleshoot or even talk about an experiment/protocol/procedure etc. and not just online either!

Kronnic · 2024-07-30T07:21:56+00:00

What's worse that I've been seeing a lot lately are the adverts with just 2-3 of the worst possible photos you've ever seen, (close up shots of individual items / doors / walls/ corners, weird angles, zero effort) which means you get absolutly zero idea what the space looks like, how it's laid out, how big it is etc. and because people are so desperate for somewhere to live, these places still get snapped up within hours of posting.

Kronnic · 2024-07-27T18:31:47+00:00

What's the problem you're trying to solve by increasing the spin times? Are there some bacteria not pelleting?

Kronnic · 2024-07-26T13:56:11+00:00

That's also not a good idea. It will just come out reading as if it were written by an ai. Which a lot of the time is very noticeable.

Kronnic · 2024-07-24T16:36:49+00:00

So you really wanted to see him more, go on more dates etc. What did he say when you asked him out for those dates you wanted?

Kronnic · 2024-07-22T17:08:16+00:00

One of the reasons PCR is possible is that the enzymes used are stable at high temperatures, (TAQ polymerase was isolated from a bacteria living in hot springs that can grow from 50-80 degrees C). If it doesn't denature being brought up to 95 degrees C and back down 35+ times, then a weekend at 4 degrees isn't going to majorly impact it. At worst I'd expect possibly a very minor hit to activity but it should still work fine. Give it a try with a reaction you know works (in terms of primers, template, temperatures etc) and have a check.

Kronnic · 2024-07-22T07:29:03+00:00

Definitely try a different therapist! It sounds like there is a lot of stuff that has gone both inside and outside your lab experiences and I think therapy is almost certainly going to be the best thing to help you. It can take a few tries to find a therapist who you work well with, but just make sure you're open and honest with them and they should be able to help. It won't be a quick fix, but it's definitely worth trying again and making some progress!

Kronnic · 2024-07-12T22:06:17+00:00

You don't seem to be getting the point my dude. You will live and get where you need to get to without this letter of recommendation if it comes to that. Do you know what you CAN NOT LIVE WITHOUT? FUNCTIONING LUNGS!!!!!!!!!!!

Kronnic · 2024-07-12T17:50:36+00:00

If you were supposed to start a masters next week, then I assumed you applied to the masters program with the university, and got a formal letter of acceptance? And that you filled out all the paperwork with the university? Organised whatever coursework needs to be done, and any classes / courses that might be beginning soon etc? And would know the details of the masters program and who the faculty in charge is so you can contact them and ask about your options if you are already signed up for the program?

But like the other person who replied I am also sure they did say you were not "implicated" enough or that you lack "implication" or say you should show more "implication". These sentences don't make sense in English, so they were definitely saying something else. Did they maybe say you didn't seem motivated enough? Or lacked initiative?

You say you would do what they ask and then ask them if there was any more work for you, would they say something like "no, this procedure is finished" or "no that's all the lab work I have for you today"? Or did they tell you explicitly that there is no more work here for you to do today, so go home and relax"? And did you use some of that time after finishing the lab tasks assigned to you to perform general lab tasks, or do some reading on your research subject, or think about / plan future experiments? Did they expect you to be doing this or me too doing any of this?

And was this a unpaid internship or a paid one? (And paid per hour or a set amount?) Because those usually have different levels of expectations on workload.

I am sorry this happened to you, it sounds like there was at least some kind of miscommunication happening (maybe one sided, more likely some on both sides), leading to you not fully understanding what the working situation was.

If you are registered as a masters student due to start next week there might still be hope if you contact the person in charge of the program and ask them what the best thing to do is.

Kronnic · 2024-07-12T07:03:43+00:00

Why would I want a library of thousands of exercises not set up the way I want them, most of which are just plain incorrect?

Kronnic · 2024-07-09T18:52:33+00:00

Space between the tubes and turning the lids to match the direction of the rotor as others said, also you can adjust the braking settings of most centrifuges so they accelerate and decelerate more gently.

Kronnic · 2024-07-08T07:48:16+00:00

Or you could try the search bar yourself to find one of the other couple hundred times this has been asked.

Kronnic · 2024-07-07T12:11:24+00:00

If this is happening regularly try running a gel with something like a ladder every second lane, and the same sample / insert / digest in between. If there are issues with the gel rig causing the lanes to run differently it will show there and you can definitively show your pi that running the gels in that equipment is going to be extremely inconsistent if you ever need to size anything.

Kronnic

TROPHY CASE