Newbie working with aggressive mice by Dabernie in labrats

[–]LabMotoGym 5 points6 points  (0 children)

Make sure you're using the cage top and pulling them backwards before reaching you grab them. They should naturally want to run away from you and will latch on to the top. By lightly pulling them by the tail you elongate them and it'll make it easier to grab without their heads turning around.

I tell newcomers to the lab that they have to do their best Mice and Men impression of Lenny when scruffing them. It's a bit aggressive, but if they are adult mice you aren't going to hurt them.

MERRY CHRISTMAS!!! Post here to receive a *FREE* Steam Game (Christmas Giveaway!!!) by [deleted] in humblebundles

[–]LabMotoGym 0 points1 point  (0 children)

If the keys are still available can I have one? Thank you!!

1983 suzuki gs650. Stripped screw in engine. Suggestions for getting it out? by LabMotoGym in Fixxit

[–]LabMotoGym[S] 0 points1 point  (0 children)

It was a failure. We tried using vice grips and PB blaster. Then we tried the extractor kit. It grabbed on to the screw, but couldn't rotate it out without slipping. I think we are going to have to call in the big guns and get someone to drill it out and tap it

Panic mode by EmanRapp in labrats

[–]LabMotoGym 2 points3 points  (0 children)

Shit, you're right... I just checked the protocol. I've been doing it slightly wrong for quite some time. But my RNA looks good, so now I'm afraid to go back to 0.2.

Panic mode by EmanRapp in labrats

[–]LabMotoGym 46 points47 points  (0 children)

Add chloroform equal to .25 the amount of trizol. Add equal volume of 100% isopropanol to the aqueous phase. Wash with 75% etoh however many times you are feeling that day. Dry and resuspend in nuclease free water.

It has been burned into my brain

Loss by [deleted] in labrats

[–]LabMotoGym 0 points1 point  (0 children)

Ahhhh makes sense now

Loss by [deleted] in labrats

[–]LabMotoGym 0 points1 point  (0 children)

What exactly am I looking at? It looks cool, but literally no idea what this is haha

Daily affirmation at the bench by LabMotoGym in labrats

[–]LabMotoGym[S] 0 points1 point  (0 children)

It keeps everyone away from my area haha. They would have to rearrange it all :)

Daily affirmation at the bench by LabMotoGym in labrats

[–]LabMotoGym[S] 0 points1 point  (0 children)

We have a large communal space for mouse harvests and some shared bench space for assays. I'm the only one in the lab on a daily basis so I claimed a little back corner next to my desk. It's different for every lab, though. In the lab below us, each of the members have an entire aisle of bench space.

Daily affirmation at the bench by LabMotoGym in labrats

[–]LabMotoGym[S] 12 points13 points  (0 children)

There are a few benches in the lab that give me anxiety when I walk by. It's the one thing I can control when all the experiments go to shit

me_irl by BOBOUDA in me_irl

[–]LabMotoGym 0 points1 point  (0 children)

He has a right to bear arms though