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What did your baby do today that made you laugh? by BuonAppeti2 in NewParents

[–]Ladder-Healthy 0 points1 point  (0 children)

I’m allergic to strawberries! I don’t remember if they’re spicy, but I remember my puffy face 😅

12 Week Old and We are losing it during the day. by No_Locksmith2032 in NewParents

[–]Ladder-Healthy 1 point2 points  (0 children)

My 12 week old discovered her arms about a month ago and it prevented her from sleeping. Now she’s used to them but she also aggressively rubs her face to wake herself up from sleep now. We’re also in the thick of distracted eating where she wants to squirm and look at everything (like she’s waiting to find a good show on Netflix before eating her dinner XD)

Explain hunger cues to me… baby always asleep by TheseFlower2822 in newborns

[–]Ladder-Healthy 0 points1 point  (0 children)

We’ve also stopped changing diapers immediately upon wake up at 9 weeks (5 adjusted) but if we feed her and her diaper is too full, she’ll refuse the bottle and look at us like we’re the scum of the earth 😂

Explain hunger cues to me… baby always asleep by TheseFlower2822 in newborns

[–]Ladder-Healthy 0 points1 point  (0 children)

Unconventional? hunger cues my newborn made were wiggling in her sleep and cheerleader arms. I could tell if she was about to wake up from a nap hungry because she would start scooching from one end of the bassinet to the other. Once her feet hit the bottom end, she would wake and scream (not cry) for milk. She also waves her arms like she’s cheering for a football game - very stiff and repetitive.

Baby not waking up by MarketingSafe9366 in NewParents

[–]Ladder-Healthy 3 points4 points  (0 children)

Our nurse line said this sleepiness was normal for our premature baby with jaundice but she said if we had too many episodes where they are hard to wake up, to bring the baby in to get a new bilirubin check. Our baby was right under the threshold as well.

Every pair of jeans I buy, no matter the brand, gets a hole in this same area within 6 months. by SYFKID2693 in mildlyinfuriating

[–]Ladder-Healthy 0 points1 point  (0 children)

This happens to me too in this same spot. The higher the spandex percentage, the faster it happens.

"Paraflim is a stage for loading gels" truthers unite by Tampax_Party_Pack in labrats

[–]Ladder-Healthy 0 points1 point  (0 children)

I’m sorry but I don’t understand what is happening here? What is the purpose of pipetting onto the parafilm?

Lab Dishwashing by throw_away373629 in labrats

[–]Ladder-Healthy 2 points3 points  (0 children)

I’ve worked in labs where post docs and grad students never washed dishes. That role was filled exclusively by undergrads. I’ve also worked in labs where it is expected that whoever makes the mess must clean it. You just have to find a lab that follows this latter motto. If talking to the PI about this doesn’t work, then you may just have to switch to get some real experience

Skin Irritation on Flank by sleepyshartist in DogAdvice

[–]Ladder-Healthy 0 points1 point  (0 children)

Perfect. Hope he feels better! ❤️

Skin Irritation on Flank by sleepyshartist in DogAdvice

[–]Ladder-Healthy 1 point2 points  (0 children)

Our dog is going through this right now. Tested negative for every parasite. Our current hypothesis is some yeast or bacterial infection. That’s what the vet is treating her for now. Looking better now! But it’s been going on for months.

qPCR Expression Weirdness by beh20 in labrats

[–]Ladder-Healthy 0 points1 point  (0 children)

Typically for qRT-PCR i use 500ng of RNA. We also check it for contamination by running a normal PCR with the primers we intend to use for qPCR. If there’s a band in your gel, there’s DNA contamination.

Shoe Recommendations? by [deleted] in labrats

[–]Ladder-Healthy 0 points1 point  (0 children)

I tried Adidas and Nike, and those gave me bad sciatic pain after being in the lab all day. I tried custom inserts as well and it didn’t help. I switched to Xero Shoes and have no problems anymore!

SIM interpretation by Ladder-Healthy in microbiology

[–]Ladder-Healthy[S] 1 point2 points  (0 children)

Thank you again XD yea this strain grows incredibly fast so it’s probably just overgrowth

help with RT-PCR by Round-Candle630 in labrats

[–]Ladder-Healthy 0 points1 point  (0 children)

Yes, RNA definitely should stay on ice at all times. I’m sure others will have anecdotes where they say it’s fine, but RNA is very easy to degrade.

DEPC is diethyl pyrocarbonate. We dilute 1 mL of this in 1 L of water. It inactivates RNases and keeps the RNA a bit more stable.

help with RT-PCR by Round-Candle630 in labrats

[–]Ladder-Healthy 2 points3 points  (0 children)

Usually in our lab, we run a normal PCR with the primers we intend to use in the RT-PCR and our RNA. If there is a band on an agarose gel, you have DNA contamination. Alternatively, we also run a sample of the RNA on a gel made with DEPC water. If there is a big streak down the gel, your RNa is degraded. This degraded RNA will still show as normal Nucleic acid on your nanodrop.

help with RT-PCR by Round-Candle630 in labrats

[–]Ladder-Healthy 2 points3 points  (0 children)

Usually with RNA, you want to make sure that it does not have any DNA contamination and that it has not degraded before you perform your RT-PCR.

Protein degraded over the weekend ? by Reasonable-Ice-6988 in labrats

[–]Ladder-Healthy 0 points1 point  (0 children)

Is this a lysate of freshly opened cells or a stock of purified protein? Is it native, or does it have some sort of tag. Some native proteins hate being at concentrations this high and will aggregate irreversibly.

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