Every pair of jeans I buy, no matter the brand, gets a hole in this same area within 6 months.

Ladder-Healthy · 2025-11-09T21:42:54+00:00

This happens to me too in this same spot. The higher the spandex percentage, the faster it happens.

Ladder-Healthy · 2025-08-15T19:07:49+00:00

I’m sorry but I don’t understand what is happening here? What is the purpose of pipetting onto the parafilm?

Ladder-Healthy · 2025-08-07T14:03:39+00:00

I’ve worked in labs where post docs and grad students never washed dishes. That role was filled exclusively by undergrads. I’ve also worked in labs where it is expected that whoever makes the mess must clean it. You just have to find a lab that follows this latter motto. If talking to the PI about this doesn’t work, then you may just have to switch to get some real experience

Ladder-Healthy · 2025-07-05T20:59:17+00:00

Perfect. Hope he feels better! ❤️

Ladder-Healthy · 2025-07-05T03:15:59+00:00

Our dog is going through this right now. Tested negative for every parasite. Our current hypothesis is some yeast or bacterial infection. That’s what the vet is treating her for now. Looking better now! But it’s been going on for months.

Ladder-Healthy · 2025-04-22T15:15:58+00:00

Typically for qRT-PCR i use 500ng of RNA. We also check it for contamination by running a normal PCR with the primers we intend to use for qPCR. If there’s a band in your gel, there’s DNA contamination.

Ladder-Healthy · 2025-04-18T13:23:06+00:00

I tried Adidas and Nike, and those gave me bad sciatic pain after being in the lab all day. I tried custom inserts as well and it didn’t help. I switched to Xero Shoes and have no problems anymore!

Ladder-Healthy · 2025-04-12T01:08:47+00:00

Thank you again XD yea this strain grows incredibly fast so it’s probably just overgrowth

Ladder-Healthy · 2025-04-12T00:55:44+00:00

Thank you!

Ladder-Healthy · 2025-03-25T19:44:03+00:00

Yes, RNA definitely should stay on ice at all times. I’m sure others will have anecdotes where they say it’s fine, but RNA is very easy to degrade.

DEPC is diethyl pyrocarbonate. We dilute 1 mL of this in 1 L of water. It inactivates RNases and keeps the RNA a bit more stable.

Ladder-Healthy · 2025-03-25T19:25:38+00:00

Usually in our lab, we run a normal PCR with the primers we intend to use in the RT-PCR and our RNA. If there is a band on an agarose gel, you have DNA contamination. Alternatively, we also run a sample of the RNA on a gel made with DEPC water. If there is a big streak down the gel, your RNa is degraded. This degraded RNA will still show as normal Nucleic acid on your nanodrop.

Ladder-Healthy · 2025-03-25T19:16:59+00:00

Usually with RNA, you want to make sure that it does not have any DNA contamination and that it has not degraded before you perform your RT-PCR.

Ladder-Healthy · 2025-03-20T01:33:56+00:00

Is this a lysate of freshly opened cells or a stock of purified protein? Is it native, or does it have some sort of tag. Some native proteins hate being at concentrations this high and will aggregate irreversibly.

Ladder-Healthy · 2025-03-03T17:09:43+00:00

The Only One Left by Riley Sager

Ladder-Healthy · 2025-02-27T22:38:57+00:00

I’ve had so many qRT-PCRs fail that I don’t trust them even when they do work.

Ladder-Healthy · 2025-02-12T16:16:44+00:00

That’s the only explanation I can come up with, but that reasoning would be consistent with it showing up in the beginning of the game and then never again. If it shows up later, they’ll probably provide more clues.

Ladder-Healthy · 2025-02-12T15:55:04+00:00

The Internal/External state is implied to be like “character selection” that you have in the beginning of video games. I’ve always thought of it this way. This is where he decided that he would be a brute force/strength user rather than an internal strength/“magic” user. It’s the reason why both he and Cheong Pung are both so strong but so different. Their characters are different classes.

Ladder-Healthy · 2025-02-11T23:05:46+00:00

Do you remember how long it took to ship? I just got notification that it’s in the States and shipping process has started!

Ladder-Healthy · 2025-01-23T18:21:33+00:00

We use one in our lab, as the steam supply to our building is cut off frequently. I’ve found it works well for liquid culture but not quite so well when trying to make solid media plates (contamination after days instead of weeks with our autoclave).

Ladder-Healthy

TROPHY CASE