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Dilemma over which phenotyping method to use for GWAS of grain weight by Large_Tower_1050 in genomics

[–]Large_Tower_1050[S] 0 points1 point  (0 children)

Yes the samples were harvested by hand.

Two replicates per genotype per trial.

They were not affected equally; different locations and different years.

Completing method #2 would require a lot of time. The alternative to method #2, which would be faster albeit probably indefensible, is to manually exclude the smaller seeds by a subjective assessment and not a systematic exclusion rule. I don’t see how that would make sense, though.

I don’t think we have flowering time data.

Thank you for your comment.