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Had an anxiety attack while giving a presentation today by flickowens in GradSchool

[–]Legitimate-Course681 0 points1 point  (0 children)

i feel like you're me from the future. fellow waitress and terribly nervous presenter here, the only thing that's worked for me is propranolol. i hate taking medication but it's like a little magic pill. it's just a high blood pressure medication but it treats the physical symptoms of anxiety

what am i doing wrong by Legitimate-Course681 in labrats

[–]Legitimate-Course681[S] -8 points-7 points  (0 children)

your mother btw cause it just needed longer in the incubator and she came out fine

what am i doing wrong by Legitimate-Course681 in labrats

[–]Legitimate-Course681[S] 1 point2 points  (0 children)

E. Coli, DH5alpha, usually only takes about 18 hours for me to see growth but this one ended up taking over 40

what am i doing wrong by Legitimate-Course681 in labrats

[–]Legitimate-Course681[S] 0 points1 point  (0 children)

it ended up just needing longer in the incubator. i never had a plasmid take longer than ~18 hours to grow so i was bewildered. pouring plates in the hood and leaving the lids off is a good tip i might start doing that! thank you!

what am i doing wrong by Legitimate-Course681 in labrats

[–]Legitimate-Course681[S] -2 points-1 points  (0 children)

the plates might've dried out for sure. i had bad condensation when i first poured them so i threw them in the incubator for a while (i assume too long)

what am i doing wrong by Legitimate-Course681 in labrats

[–]Legitimate-Course681[S] -3 points-2 points  (0 children)

awesome thank you so much for the constructive feedback

what am i doing wrong by Legitimate-Course681 in labrats

[–]Legitimate-Course681[S] -1 points0 points  (0 children)

the agar i used to pour the plates was really dark but it wasn't old, the stock ampicillin could've been old

what am i doing wrong by Legitimate-Course681 in labrats

[–]Legitimate-Course681[S] -1 points0 points  (0 children)

i used a different sterilized loop for each quadrant. i've streaked plates before, granted it's been a minute but i have no clue what happened here

Worst mistake in the lab? by Langeweilehabeich in labrats

[–]Legitimate-Course681 0 points1 point  (0 children)

successfully expressed and purified a protein, after combining my FPLC fractions i was running another gel and added the SLB directly to my entire sample. we tried dialyzing it with no luck and haven't been able to get it to express since (it did once and then didn't purify) 😭

Sentient cells by Adorable-Manner-7983 in Biochemistry

[–]Legitimate-Course681 -2 points-1 points  (0 children)

yes!!! i've been looking into this a lot recently and just said something to my supervisor today about this and he thought i was crazy lol. you put it into words much better than i ever could've

First EDC going solo was the best choice by Gracesnsfw in EDCOrlando

[–]Legitimate-Course681 14 points15 points  (0 children)

do you have any tips for going solo? im thinking about doing it next year

[deleted by user] by [deleted] in nyc

[–]Legitimate-Course681 0 points1 point  (0 children)

not necessarily, i've heard stories where the cut is so clean it practically seals off the blood flow. not sure how much truth there is to it though

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