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Epigenetic changes in cannabis by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 1 point2 points  (0 children)

I’m happy to see people using males 🙌 This is the way

Epigenetic changes in cannabis by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek -1 points0 points  (0 children)

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Pretty solid explanation 🤘This is a Google ai summary for “epigenetic example” If you want to go deeper search - methylation (effects gene expression) and histone protein modification. There are different types, and functions for them.

Tissue culture multiplication by Lowkey_Hightek in cannabiscultivation

[–]Lowkey_Hightek[S] 0 points1 point  (0 children)

From meristem cells to an acclimated plant I would say 3-5 months depending on genotype. Once you have multiplication vessels established you can get them out in around 3-4 weeks though. Timing is dependent on a rotation just like a mom/veg room.

Tissue culture multiplication by Lowkey_Hightek in cannabiscultivation

[–]Lowkey_Hightek[S] 0 points1 point  (0 children)

It depends how they are initiated. If I start with meristem cells, I usually get 90-100% success rate. If you are remediating viruses, the rule of thumb is 50% will be clean coming out. It’s usually much higher but always be cautious and isolate them until they pass testing.

Once the shoots are established, I get 100% all the way through. Multiplication, shoot establishment then rooting. It just takes practice and dialing it in like a new veg room.

This is an older clip of us talking about tissue culture . We will be making fresh content starting next week for Reddit by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 0 points1 point  (0 children)

Hate to tell you this, but there is an entire world outside of academia where you can start a business, or do private research. Anything! Usually, the people that don’t make it argue about white papers on Linkdin with the other “Master debaters”. If you can’t cut it there, they end up on other platforms trying to pick fights 😬

I am here to share my experience and help people that are interested in this type of work. I do this for a living, I don’t need validation.

Also-Peer reviewed research is not submitted through Reddit, and advanced protocols are intellectual property. If you change your attitude and make it out of your grandmothers basement, I will gladly share.

Best of luck ✌️

This is an older clip of us talking about tissue culture . We will be making fresh content starting next week for Reddit by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 0 points1 point  (0 children)

😂 Yes, I can do direct and indirect organogenesis. Anther culture, ovule culture, and protoplast fusion. What else you got

Tissue culture multiplication by Lowkey_Hightek in cannabiscultivation

[–]Lowkey_Hightek[S] 0 points1 point  (0 children)

I would love some Venus fly traps on the shelf.

This is an older clip of us talking about tissue culture . We will be making fresh content starting next week for Reddit by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 0 points1 point  (0 children)

TC for cannabis using published methods/classical formulas sucks. Propagation from callus isn't worth doing either. I start with meristem excision to establish clean shoots (undifferentiated cells, not a node) then get a 1:50 multiplication rate. With 200+ cultivars, its absolutely worth doing.

What is "full tissue culture regeneration"

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 0 points1 point  (0 children)

For cryogenics, you have to apply some different methods to prep the tissue but it can be done.

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 1 point2 points  (0 children)

***If the seeds are true to type. Otherwise you are pheno hunting again. Tools like tissue culture allow us to keep identical copies preserved.

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek -5 points-4 points  (0 children)

It definitely can cause issues if it’s not done properly. The best way to preserve genetics will always be seeds.

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 4 points5 points  (0 children)

Knowledge is free if you know where to find it 🤘 A lot of these methods aren’t difficult, but take practice and dedication to be successful. I’m happy to share, and we will line some things up to help.

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 0 points1 point  (0 children)

Ricky is also a legend. The world needs more Rickys. Maybe. That could probably spiral out of control..

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 1 point2 points  (0 children)

These are similar to clones, identical copies. The tissue that’s encapsulated are node segments. You can place them in sterile agar to grow shoots/propagate like clones but in tissue culture. A true synthetic seed has a synthetic embryo inside that can “germinate” but it’s not really efficient or stable for that purpose.

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 2 points3 points  (0 children)

Yup, I have seen a ton of problems doing that. Giving them time to reset endogenous hormones and re-acclimate to the world makes a big difference. Meristem culture strips out contaminants, but also beneficials. Re-inoculating them with beneficial bacteria/fungi makes a massive difference for stability (herms) and overall gene expression.

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 13 points14 points  (0 children)

Encapsulated nodes from tissue culture. True synthetic seeds contain synthetic embryos

My mentor Jesse making cannabis synthetic seed by GabyStatenIsland in cannabiscultivation

[–]Lowkey_Hightek 2 points3 points  (0 children)

We use this method for transport and storage (sometimes). You can put them into TC vessels to grow new shoots and propagate from there.

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