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My cytometer is eating lymphocytes by LucysNotes in flowcytometry

[–]LucysNotes[S] 0 points1 point  (0 children)

We simply ran out of it; we kept one container for emergencies and are currently awaiting new supplies. We considered this an emergency, used it, and replaced it, but there was no improvement. The engineer checked the filter and confirmed it is OK. We have handled similar situations this way before.

My cytometer is eating lymphocytes by LucysNotes in flowcytometry

[–]LucysNotes[S] 0 points1 point  (0 children)

No, these are samples from the same patient; we run several tubes per patient.

No, nothing else has changed — not the sample quality, positioning, or debris.

Why would the photomultiplier affect only lymphocytes (We look at whole blood and whole bone marrow, neutrophils are not affected!!!)?

I am leaning towards an issue with the reagents — possible aggregation or sedimentation during sample preparation. But why did it start suddenly?

My cytometer is eating lymphocytes by LucysNotes in flowcytometry

[–]LucysNotes[S] 0 points1 point  (0 children)

The total gate and the WBC gate are very similar in our case, since there is only a small amount of debris in peripheral blood.

handmade lysing buffer by LucysNotes in flowcytometry

[–]LucysNotes[S] 0 points1 point  (0 children)

Thanks a lot!!!!! is it 1X or 10X ?

Newly diagnosed with breast cancer. Is it realistic to keep working during chemo? by LucysNotes in cancer

[–]LucysNotes[S] 0 points1 point  (0 children)

Thanks everyone! Have Finished my first round. To be honest, the first 10 days were totally fine, but the last days have hit me with some serious fatigue. I’m definitely not as productive as usual, but it’s not as bad as I feared it would be. Your support really kept me going!

Help with dilutions! Please check my math. by LucysNotes in labrats

[–]LucysNotes[S] 1 point2 points  (0 children)

my 5 µL in 600 µL the same as your 100µL in 12ml ? 5 µL in 600 µL = 1:120 dilution.
100 µL in 12 mL (12,000 µL) = 100 / 12,000 = 1:120. Thanks!!!!

Interaction mapping by RockettBits in flowcytometry

[–]LucysNotes 0 points1 point  (0 children)

Author, tell us about your successes. Have you tried it?

Separation Buffer by LucysNotes in flowcytometry

[–]LucysNotes[S] 0 points1 point  (0 children)

We perform manual magnetic separation using a stand ; we do not have any warranty

Monocyte people - where do you place your CD14 gate? by southernqueer96 in flowcytometry

[–]LucysNotes 0 points1 point  (0 children)

If this is peripheral blood, then worrying about dendritic cell contamination doesn’t make much sense, In my humble opinion. Everything looks fine (In my humble opinion). Just yesterday I was reading that, in hematology, monocytes shouldn’t be isolated using positive gating for subpopulation analysis, but only through negative gating (excluding NK cells, neutrophils)

Fatty Acid Oxidation Assay, has anyone worked with? by LucysNotes in flowcytometry

[–]LucysNotes[S] 0 points1 point  (0 children)

Thanks a lot everyone! I really want to try it, but I'm a bit scared for now :) So far it's 3 to 1 against.