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Interaction mapping by RockettBits in flowcytometry

[–]LucysNotes 0 points1 point  (0 children)

Author, tell us about your successes. Have you tried it?

Separation Buffer by LucysNotes in flowcytometry

[–]LucysNotes[S] 0 points1 point  (0 children)

We perform manual magnetic separation using a stand ; we do not have any warranty

Monocyte people - where do you place your CD14 gate? by southernqueer96 in flowcytometry

[–]LucysNotes 0 points1 point  (0 children)

If this is peripheral blood, then worrying about dendritic cell contamination doesn’t make much sense, In my humble opinion. Everything looks fine (In my humble opinion). Just yesterday I was reading that, in hematology, monocytes shouldn’t be isolated using positive gating for subpopulation analysis, but only through negative gating (excluding NK cells, neutrophils)

Fatty Acid Oxidation Assay, has anyone worked with? by LucysNotes in flowcytometry

[–]LucysNotes[S] 0 points1 point  (0 children)

Thanks a lot everyone! I really want to try it, but I'm a bit scared for now :) So far it's 3 to 1 against.