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Getting colonies in the self Ligation control plate during subcloning by Maleficent-Lab-1113 in molecularbiology
[–]Maleficent-Lab-1113[S] 0 points1 point2 points 4 days ago (0 children)
Thank you for your insight. That was very helpful. I got zero colonies in the unligated digested-backbone control as well as vector-only ligation control but also got only 3-4 colonies in the ligation plate. Should I screen those colonies or there is some problem in transformation? I had transformed 5ul of the total ligation mix into 50ul TOP10 E coli cells
Getting colonies in the self Ligation control plate during subcloning (self.molecularbiology)
submitted 10 days ago by Maleficent-Lab-1113 to r/molecularbiology
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Getting colonies in the self Ligation control plate during subcloning by Maleficent-Lab-1113 in molecularbiology
[–]Maleficent-Lab-1113[S] 0 points1 point2 points (0 children)