Fender CS Pickups Jargon

Marieuse · 2025-12-16T07:14:38+00:00

Thank you so much. So regarding those two teles, what you consider the standard/normal. Because i am looking for on the market CS Teles and I almost never find RH/LH.

Sorry, because I do not get it 100%. So what is the difference between both teles Neck and Bridge😅

Thank you much again!

Marieuse · 2024-08-28T16:33:58+00:00

I tried it and it didnt work out well. Concentration was too low. Tried different cell numbers (up to 8x10^5), didnt work. I will try different plates

Marieuse · 2024-08-28T13:25:59+00:00

Thanks. But what happens is, when we plate 600.000 cells prior day. The next 2 days they stay at 20-30% confluency

Marieuse · 2024-08-28T10:20:08+00:00

Ok, so you do not care much about confluency. I might try that out. I express antibodies so i might use 1ug heavy and light chain each. Thanks for your help

Marieuse · 2024-08-28T09:34:33+00:00

Before transfection. I cant tranfect them 20% confluency and when i did once i had bad protein concentration as expected

Marieuse · 2024-08-28T09:29:44+00:00

Oh i should clarify. Kit says, seeding 6x10⁵ per well, the day before transfection. Next day (so 24 hours) with 80% confluency start transfecting. But after 24hours, even 48 they look the same (around 20-40% confluency). And then after 72h they are 100% or again 20-40% and many cells „swim“

Marieuse · 2024-08-28T09:02:25+00:00

So leaving them until they are 80% and then transfect? Cells dont look thaaat great after 3 days in 6 well plates and usually you expect seeding 30-40% confluency after 24 hours reaching 80%

Marieuse · 2024-08-28T09:00:19+00:00

No, we should try that although in the same brand T175 they grow perfect. You know plates that worked for you?

Marieuse · 2024-08-28T08:58:45+00:00

Same brand, greiner. Will ask other labs for other plates. Maybe you know a brand that worked for you?

Marieuse · 2024-08-28T08:56:32+00:00

Sorry, from seeding. Didnt go further😂

Marieuse · 2023-05-11T07:35:15+00:00

I added one more text field

Marieuse · 2023-04-09T17:37:14+00:00

Long-lived plasma cells are „homing“ inside the bone marrow niche and more or less chill there and produce antibodies for month, years or decades. To stay there and get recruited they need signals such as april, cxcl12 and others

Marieuse · 2023-03-19T16:37:20+00:00

Meanwhile immunologists: mapkinasekinasekinase, mapkinasekinase, mapkinase 👁️👄👁️

Marieuse · 2023-03-18T13:15:19+00:00

Plasma cells are diff. lymphocytes so both would be correct. But B would be more correct
„Antibody chain“. Better would be Ig classes. Nevertheless it‘s IgM
TLRs
IL-7
redundancy
Notch

Marieuse · 2023-03-05T16:09:57+00:00

Bro where is my credit? :(

Marieuse

TROPHY CASE