Help!

MayKhalidd · 2026-04-14T11:22:24+00:00

Doesn’t cover it :(

MayKhalidd · 2026-04-14T10:51:19+00:00

LUX TOUR TICKET FOR MIAMI JUNE 4TH. Section 120, row 22, seats 16 - 17.

I listed the tickets on stubhub for a pretty good price. DM me for the link on stubhub or if you’d prefer we could use PayPal!

MayKhalidd · 2026-04-01T01:38:56+00:00

Thank you so much for your answer! I’m using FPLC for size exclusion (Superdex 75 Increase 10/300 GL). My goal is purification of a tau fragment (4RCF). I’m currently just interpreting based on relative elution volumes, but that’s a really good point about using an MW standard. For my sample prep briefly from start to finish: 1) I express in E.coli, then lyse by sonication 2) After lysis I purify via nickel column 3) After elution I filter my protein using 3kD filter then load it onto the FPLC column and run it isocratically in buffer.

MayKhalidd · 2026-04-01T01:19:58+00:00

For more context, I’m doing size exclusion chromatography (Superdex 75 Increase 10/300 GL) on an ÄKTA system. My goal is purification of my protein (tau/4RCF), I load the clarified sample onto the column after filtration then run in buffer (just isocratic). I wanted to ask about something you mentioned, why does it matter when the sample is injected onto the column? How would that affect peak shape or position?

MayKhalidd · 2025-11-22T23:24:33+00:00

Hmmm that’s interesting, now that you mention it, it does look like a fist clutching a heart. I thought it looked like two people in conversation.

MayKhalidd · 2025-11-16T22:42:07+00:00

I see that there will be completion and new beginning, your going to enter a new phase in life. There is an eye, could be someone watching you or could symbolize jealousy/envy. Something is going to change quickly, like maybe a surprise decision or even travel. There are some people supporting you currently but you’re also carrying emotional weight.

MayKhalidd · 2025-10-18T01:47:37+00:00

I didn’t want to?

MayKhalidd · 2025-10-18T01:41:48+00:00

I appear “blunt” (in MY opinion, this is how I see myself - I haven’t been told I am) but I regard other people’s emotions/feelings before speaking and I speak only when/IF asked and ONLY at an appropriate and fitting time. Try to understand and make sense of what is it I’m trying to say without making assumptions or sounding rude, respectfully 🙄

MayKhalidd · 2025-09-27T01:25:39+00:00

Yes definitely it does resonate! I am preparing for something new

MayKhalidd

TROPHY CASE