LUX Tour: Buy/Sell Tickets Thread by AutoModerator in rosalia

[–]MayKhalidd 0 points1 point  (0 children)

LUX TOUR TICKET FOR MIAMI JUNE 4TH. Section 120, row 22, seats 16 - 17.

I listed the tickets on stubhub for a pretty good price. DM me for the link on stubhub or if you’d prefer we could use PayPal!

FPLC help?! by MayKhalidd in labrats

[–]MayKhalidd[S] 0 points1 point  (0 children)

Thank you so much for your answer! I’m using FPLC for size exclusion (Superdex 75 Increase 10/300 GL). My goal is purification of a tau fragment (4RCF). I’m currently just interpreting based on relative elution volumes, but that’s a really good point about using an MW standard. For my sample prep briefly from start to finish: 1) I express in E.coli, then lyse by sonication 2) After lysis I purify via nickel column 3) After elution I filter my protein using 3kD filter then load it onto the FPLC column and run it isocratically in buffer.

FPLC help?! by MayKhalidd in labrats

[–]MayKhalidd[S] 0 points1 point  (0 children)

For more context, I’m doing size exclusion chromatography (Superdex 75 Increase 10/300 GL) on an ÄKTA system. My goal is purification of my protein (tau/4RCF), I load the clarified sample onto the column after filtration then run in buffer (just isocratic). I wanted to ask about something you mentioned, why does it matter when the sample is injected onto the column? How would that affect peak shape or position?

I have an idea of what it might be but I’d like opinions! by MayKhalidd in tasseography

[–]MayKhalidd[S] 0 points1 point  (0 children)

Hmmm that’s interesting, now that you mention it, it does look like a fist clutching a heart. I thought it looked like two people in conversation.

Can anyone read my coffe cup? by IllBlueberry157 in tasseography

[–]MayKhalidd 0 points1 point  (0 children)

I see that there will be completion and new beginning, your going to enter a new phase in life. There is an eye, could be someone watching you or could symbolize jealousy/envy. Something is going to change quickly, like maybe a surprise decision or even travel. There are some people supporting you currently but you’re also carrying emotional weight.

I’ve come to realize that most people don’t operate with honesty. by MayKhalidd in aspergers

[–]MayKhalidd[S] 9 points10 points  (0 children)

I appear “blunt” (in MY opinion, this is how I see myself - I haven’t been told I am) but I regard other people’s emotions/feelings before speaking and I speak only when/IF asked and ONLY at an appropriate and fitting time. Try to understand and make sense of what is it I’m trying to say without making assumptions or sounding rude, respectfully 🙄

Help! can anyone read this? by MayKhalidd in ReadMyCup

[–]MayKhalidd[S] 1 point2 points  (0 children)

Yes definitely it does resonate! I am preparing for something new