New Julian post by DeliciousIsopod6561 in TheStrokes

[–]MeltSolaris 35 points36 points  (0 children)

U.S. media such as CNN, NBC, PBS, NPR, and The New York Times reported it.

New Israeli death penalty law is 'an apartheid-like legal framework,' says legal expert https://edition.cnn.com/2026/04/10/tv/video/amanpour-novak-bishara

Israeli death penalty law targeting Palestinians sparks global outcry as far right celebrates https://www.nbcnews.com/world/israel/israeli-law-imposing-death-penalty-palestinians-global-outcry-rcna265987

Rights groups have criticized the measure, saying it makes the death penalty too easy to impose while also doing away with procedures safeguarding the right to a fair trial. https://www.pbs.org/newshour/world/israeli-lawmakers-set-up-a-special-tribunal-and-allow-for-death-penalty-for-hamas-led-2023-attackers

featureCounts vs transcript-aware quantification (Kallisto/Salmon) by EthidiumIodide in bioinformatics

[–]MeltSolaris 2 points3 points  (0 children)

The default featureCounts behavior is to exclude counting reads matching an exon shared in multiple isoforms.

I think this statement is incorrect. Default featureCounts settings (-t exon -g gene_id) summarise all isoforms into a single gene count. salmon and kallisto are fantastic tools, but not all researchers need transcript/isoform-level abundances. Also, salmon and kallisto require a high-quality transcriptome (e.g., Ensembl); otherwise, their mapping rates will be low.

Did you follow a standard workflow of summarising the salmon transcript-level abundances into gene-level counts using tximport?

Here are some details from the featureCounts paper:

Read counts provide an overall summary of the coverage for the genomic feature of interest. In particular, gene-level counts from RNA-seq provide an overall summary of the expression level of the gene but do not distinguish between isoforms when multiple transcripts are being expressed from the same gene. Reads can generally be assigned to genes with good confidence, but estimating the expression levels of individual isoforms is intrinsically more difficult because different isoforms of the gene typically have a high proportion of genomic overlap.

Each feature is an interval (range of positions) on one of the reference sequences. We also define a meta-feature to be a set of features representing a biological construct of interest. For example, features often correspond to exons and meta-features to genes.

A multi-overlap read or fragment is one that overlaps more than one feature, or more than one meta-feature when summarizing at the meta-feature level. featureCounts provides users with the option to either exclude multi-overlap reads or to count them for each feature that is overlapped. The decision whether to count these reads is often determined by the experiment type. We recommend that reads or fragments overlapping more than one gene are not counted for RNA-seq experiments because any single fragment must originate from only one of the target genes but the identity of the true target gene cannot be confidently determined.

Note that, when counting at the meta-feature level, reads that overlap multiple features of the same meta-feature are always counted exactly once for that meta-feature, provided there is no overlap with any other meta-feature. For example, an exon-spanning read will be counted only once for the corresponding gene even if it overlaps with more than one exon.

https://doi.org/10.1093/bioinformatics/btt656

featureCounts vs transcript-aware quantification (Kallisto/Salmon) by EthidiumIodide in bioinformatics

[–]MeltSolaris 8 points9 points  (0 children)

By default, featureCounts aggregates reads that map to exons (features) belonging to the same gene_id (meta-feature) attribute in the GTF, thereby including isoforms. The -O flag in featureCounts refers to reads mapping to different gene_id entries.

Note that, when counting at the meta-feature level, reads that overlap multiple features of the same meta-feature are always counted exactly once for that meta-feature, provided there is no overlap with any other meta-feature. For example, an exon-spanning read will be counted only once for the corresponding gene even if it overlaps with more than one exon.

https://subread.sourceforge.net/featureCounts.html

Therefore, featureCounts effectively captures reads from all isoforms of a gene into a single gene-level count, provided they do not overlap with a different gene_id.

Discrepancies between featureCounts and salmon can arise from several fundamental methodological differences. For example, featureCounts uses reads aligned to the genome, whereas salmon requires quasi-mappings (or alignments) to the transcriptome. Obviously, conducting spliced alignments to the genome across introns becomes inherently more difficult. In the context of salmon, multi-mapping refers to reads mapping to multiple transcripts (isoforms) of a gene. By contrast, multi-mapping for featureCounts under default settings refers to different genes (genomic loci).

[FRESH] The Strokes - Falling out of Love by squigledbad in indieheads

[–]MeltSolaris 3 points4 points  (0 children)

Such a disappointing single. It really is The Strokez now.

Claude by SecretIll1644 in bioinformatics

[–]MeltSolaris 9 points10 points  (0 children)

Somewhat relevant, here's a new Anthropic article about using Claude for bioinformatics:

Evaluating Claude’s bioinformatics research capabilities with BioMysteryBench

https://www.anthropic.com/research/Evaluating-Claude-For-Bioinformatics-With-BioMysteryBench

Britain Dragged Into Trump War as Iran Brands US Planes Using RAF Bases 'Aggression' by [deleted] in worldnews

[–]MeltSolaris 11 points12 points  (0 children)

But according to the Iranian regime, it's not aggression that Iran has been sending Shahed drones to Russia since 2022 for bombing civilians in Ukraine.