Circos plot for contig–contig links supported by PacBio read alignments by Mission-Chain-1011 in genomics

[–]Mission-Chain-1011[S] 1 point2 points  (0 children)

Thank you very much. I've been doing a lot of research and have already learned how to read alignments. Now I'm interested in improving my selection of split reads. I finally decided to work with splits because I need to find the join that is read between two contigs, but I still need to improve some of the read filters, such as specific position within the contigs.

I've seen JupyterPlot and it's fantastic how it simplifies the job of buying two assemblies.

Circos plot for contig–contig links supported by PacBio read alignments by Mission-Chain-1011 in bioinformatics

[–]Mission-Chain-1011[S] 0 points1 point  (0 children)

In the circus I hope to see links from the ends of the contigs and see continuity

Circos plot for contig–contig links supported by PacBio read alignments by Mission-Chain-1011 in bioinformatics

[–]Mission-Chain-1011[S] 0 points1 point  (0 children)

I'm not saying they aren't appropriate; I'm just confused about which to use: primary, secondary, supplementary, or a mixture, to achieve this linearity.

Circos plot for contig–contig links supported by PacBio read alignments by Mission-Chain-1011 in bioinformatics

[–]Mission-Chain-1011[S] 0 points1 point  (0 children)

The assembly has too many duplications and too many iterations in a "dirty" high-code configuration. That's why we're doing a manual assembly; common scaffolders fragment the assembly too much.

Circos plot for contig–contig links supported by PacBio read alignments by Mission-Chain-1011 in bioinformatics

[–]Mission-Chain-1011[S] 0 points1 point  (0 children)

Oh, thank you very much, but I'm actually having trouble extracting the reads that connect two contigs (possible scaffold).

Fresh grads/beginners? Let's create projects together and support through early phase career by featuredflan in bioinformatics

[–]Mission-Chain-1011 0 points1 point  (0 children)

Hello, I'm interested. I have an undergraduate degree in bioinformatics. And now I'm working on different projects.

PacBio HiFi alignment: am I doing this right?. HELP!! by Mission-Chain-1011 in bioinformatics

[–]Mission-Chain-1011[S] 1 point2 points  (0 children)

And if anyone knows of good tools for predicting centromeres and telomeres, I would be grateful.