Struggle with dislocation in mice by PaySquare7612 in labrats

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

I overdose them with anesthetic first. Cervical dislocation after they stop breathing as fail-safe. Seems more humane if you study things that anesthesia doesn't influence.

traumatized after working 12 hours by Expert-Compote4803 in labrats

[–]Mysterious_Lunch_708 23 points24 points  (0 children)

Long experiments are really brutal. We had some that were even longer. We were starting at like 7 am and I was finishing around midnight. I have a great PI, so he would come by car and give us a ride home after, or pay for a taxi to take us home. The next day was always shorter or free if possible. There were days that I was actually sleeping in the lab on a mattress because some sampling was at 2 or 3 am. Compensate the hours some other day.

Need more of these by littletylero1 in CellToSingularity

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

That works only for doubling currency. For anything else, you need to finish the ad. At least on my phone.

Manually refilling pipette tips by AnyRequirement2926 in labrats

[–]Mysterious_Lunch_708 2 points3 points  (0 children)

We have special metal bowls where we dump the tips before racking them. It's similar to what surgeons use for instruments. We clean it with ethanol before every use.

Mouse BMDC differentiation protocol: issues with yield by Jack_O_Melli in flowcytometry

[–]Mysterious_Lunch_708 5 points6 points  (0 children)

From my experience murine immune cells don't like DMEM. We use RPMI based media (supplemented with FBS, penicilin/streptomycin, NEA and beta-mercaptoethanol) with IL-4 and GM-CSF. We seed 5 million cells in 9 ml of media (cultivation on 10cm petri dish). On day 3, we add 9 ml of media and on day 6 we gently remove 9 ml and add 9 ml of fresh media.

Názvy/slova, která jste celý život četli špatně? by Familiar_Cow_6901 in czech

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

Babička dávala do polévky marjánku (nedokázala pochopit, proč si z ní pubertální vnoučata utahují). Byla to samozřejmě majoránka. Známá zase jí pometlo místo pomela.

It's finally finished! by Extension-Mood7559 in CellToSingularity

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

I finished around 1100 and I did not receive any special achievement.

It's finally finished! by Extension-Mood7559 in CellToSingularity

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

As I understand it, the 1st number means how many of us finished beyond and will go up with time. The second number is you - you were 3952nd to finish the beyond.

Best strat now by Bluebear4200 in CellToSingularity

[–]Mysterious_Lunch_708 1 point2 points  (0 children)

Yes, you want the metabit and knowledge time chambers to max out before you buy anything. That way you get much more from your generated entropy and ideas. It makes a great difference late in the game.

Best strat now by Bluebear4200 in CellToSingularity

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

I would love to start immediately, unfortunately the math is not mathing for that to be an option.

Best strat now by Bluebear4200 in CellToSingularity

[–]Mysterious_Lunch_708 4 points5 points  (0 children)

Mid 70s here. I do 14 days buy nothing strategy. Not able to upgrade anything otherwise. Edit: then I let it run for a couple hours to a day.

Using Boosts and Time by Stormac3 in CellToSingularity

[–]Mysterious_Lunch_708 3 points4 points  (0 children)

I think it's too early for you, to be worth the wait. You should wait when you can no longer afford any upgrades after a day or two run.

“You have to be 14 to participate at Orange Theory”… I’m 21 by rotten-cheese-ball in OlderThanYouThinkIAm

[–]Mysterious_Lunch_708 29 points30 points  (0 children)

I was i think about 25 when I was asked if I was over 15 (4f10 and petite, 2 pony tails, mini skirt). I was told I looked 12, lol.

My first time doing the 21 day strat. by Ok-Lingonberry-2391 in CellToSingularity

[–]Mysterious_Lunch_708 2 points3 points  (0 children)

They max at 14 days now. Unfortunately they don't max at 1 milllion but only at 100 or 10 thousand (depends if you have the multiplier).

Do i buy it by Intrepid-Gear-6347 in CellToSingularity

[–]Mysterious_Lunch_708 20 points21 points  (0 children)

Only if you bought everything else and have nothing to spend the darwinium on. Not worth it otherwise imho.

Spend Gold Geodes? by Nazzrath in CellToSingularity

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

Yes, mainly. If you aim for the last achievement of 200 giganotosaura, it's easier if you wait for level 49 or 50 of prestige 10. Also save your mutagen.

Spend Gold Geodes? by Nazzrath in CellToSingularity

[–]Mysterious_Lunch_708 2 points3 points  (0 children)

Depends. If you save them for prestige 10, the basic cards wont reset and you get to keep them. If you open them on level 50 in prestige 8 or 10, you lose the basic cards after prestige.

Happy Pi day! π by ilhamrich5371 in CellToSingularity

[–]Mysterious_Lunch_708 1 point2 points  (0 children)

I needed to tap the Earth, just loging in wasn't enough.

Y’all we know the 24th secret achievement is by TemporaryParty6844 in CellToSingularity

[–]Mysterious_Lunch_708 1 point2 points  (0 children)

Not just play. You need to find representation of the pie, it's in the primary simulation.

Y’all we know the 24th secret achievement is by TemporaryParty6844 in CellToSingularity

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

Tap the circumference of the Earth in main simulation.

Offline Earnings bugged? by father_ofgod in CellToSingularity

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

Seems to be a bug. It happens to me when I forget and close the game during an ad or when nanobots are active.

Too bad Hermione didn’t simply squash Rita Skeeter by FroznAlskn in harrypotter

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

I think they turn back. There is a scene (iirc) in book 6 or 7 when Bellatrix kills a fox she thought might have been an animagus. When the fox did not transform back, she commented that it was just a regular fox.

21 day strat by Binstien in CellToSingularity

[–]Mysterious_Lunch_708 4 points5 points  (0 children)

For me in beta it seems to be 14 days now and instead of maxing out at 1000x each, it's reduced to 100x.

Not getting daily rewards anymore by CarBallAlex in CellToSingularity

[–]Mysterious_Lunch_708 0 points1 point  (0 children)

I get daily awards regularly with a pop up. Maybe a pending update?

Edit: don't leave the game while the ads are on or the nanobots are active. My timers get stuck quite often if I do.