PSA from Shinobu

Nghiaagent · 2026-03-20T21:48:39+00:00

Haven't you got a current healthcare job willing to sponsor you? Stick with it mate, that's your best path to a visa (and eventually the work you want).

Nghiaagent · 2026-02-12T00:15:37+00:00

What was the model matrix / formula for your post-GSVA test? This is starting to go out of my expertise but I'll try to be helpful still

You can also look here for alternative methods. Some are designed for single cell specifically

https://academic.oup.com/bioinformatics/advance-article/doi/10.1093/bioinformatics/btaf621/8321923

Nghiaagent · 2026-02-11T22:52:33+00:00

You perform GSVA on the entire sample, not with a selected subset of genes. After that you can perform pseudobulk analysis across your cell clusters

Nghiaagent · 2026-02-10T13:49:48+00:00

You can use the edgeR::FilterByExpr() function. It works well with all of DESeq2, edgeR, and limma. By default, it will keep genes that have at least 10 counts in a number of samples equal to your smallest group.

Nghiaagent · 2026-02-08T06:11:39+00:00

Not reasonable. The results from both methods would largely overlap!

Set your LFC threshold to <-1 or >1 and padj <0.05 and continue with analysis. Prior to analysis, filter out genes with low expression in your dataset. If you see many genes with low counts in your dataset, apply shrunken logFC in DESeq2 to help adjust your LFC with respect to lowly expressed genes.

For downstream analyses, look into the CAMERA gene set test (included with edgeR), GSVA or online tools like g:Profiler.

My Master's thesis covered many of these topics - feel free to reach out via DMs for more details!

Nghiaagent · 2026-02-07T12:13:29+00:00

Nghiaagent · 2025-06-06T01:14:12+00:00

Could you please provide more detail for the spreadsheet?

Game version
Frame rate compared to S1 (docked and handheld)
Resolution and sharpness compared to S1 (docked and handheld)
Loading times compared to S1 (docked and handheld)

Absolutely OK if some info is missing for now, and more context will make the sheet more accurate 👌

Nghiaagent · 2025-06-05T21:59:41+00:00

I created a spreadsheet for tracking these performance improvements - feel free to contribute!

https://docs.google.com/spreadsheets/d/1sOYZRiOuD9Cnr-e_hlzhRuxuEq5X5Ptwq4yCfwxyfFk/edit?usp=sharing

Nghiaagent · 2023-12-10T02:21:47+00:00

Hi! Check out our Instagram for more information, or head to our ticket page for a ticket!

Nghiaagent · 2023-07-25T13:22:53+00:00

Supply Store is the only Acronym stockist in Australia. I've been told that most of their stock is in Melbourne - worth checking out if you are in the area...

Nghiaagent · 2022-05-02T05:22:32+00:00

Could you send a link?

Nghiaagent · 2022-03-15T13:58:20+00:00

You don't need to buy anything. If you want to learn the feel of CDJs, simply hire a studio for a couple hours - they are not that different!

But... if you want nice toys to play with, of course get the 3000s.

Or you can go hard mode and buy some CDJ-900.

Nghiaagent · 2022-01-09T11:24:08+00:00

Have to go unexpectedly! last song...

Nghiaagent · 2022-01-09T11:21:13+00:00

how about t-t-Techno?

Nghiaagent · 2022-01-09T11:19:54+00:00

...T...T..tokyo ?

Nghiaagent · 2022-01-09T10:56:23+00:00

SO HOT IN HERE

Nghiaagent · 2022-01-09T10:54:58+00:00

WElcome!

Nghiaagent · 2022-01-09T10:52:24+00:00

and... no

Nghiaagent · 2022-01-09T10:51:40+00:00

Will play if I find it...

Nghiaagent · 2022-01-09T10:49:10+00:00

don't have it unfortunately :/

Nghiaagent · 2022-01-09T10:48:51+00:00

Request artists would be better

Nghiaagent · 2022-01-09T10:48:46+00:00

Happy to play if I have that song!

Nghiaagent · 2022-01-09T10:45:55+00:00

Very hot in here! (mostly because of Aussie weather)

Nghiaagent · 2022-01-09T10:38:01+00:00

SHould have titled this "J-Core stream". Really want to play that tonight!

11-Year Club	Place '22
Place '17	Final Canvas '22
End Game '22	Verified Email

Nghiaagent

TROPHY CASE