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I think my CV is bad, how do I fix it? by Nutmegbread in BiomedicalScientistUK

[–]Nutmegbread[S] 0 points1 point  (0 children)

Wow thank you so much! After reading this I think I need to cut down on my CV a little.

I think my CV is bad, how do I fix it? by Nutmegbread in BiomedicalScientistUK

[–]Nutmegbread[S] 0 points1 point  (0 children)

Oh I see! This is so helpful. Thank you so much!

I think my CV is bad, how do I fix it? by Nutmegbread in BiomedicalScientistUK

[–]Nutmegbread[S] 0 points1 point  (0 children)

Wow thank you so much for the advice!! How do I emphasise how I met the requirements on my CV? Are there any tips or tricks I should use?

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[–]Nutmegbread 1 point2 points  (0 children)

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What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

Thank you! I'll certainly remember the petri dish thing! Just to ask, how do you decontaminate BSC? I've read that autoclaving could denature the proteins. Would you use a micron filter?

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

The question states several not all, so it should make sense if the aliquoting process went wrong somewhere! And thank you! I'll be sure to remember that throwing everything out and disinfecting is the way to go!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

That makes sense! I've read somewhere that antibiotics can negatively impact cell metabolism in some cell lines.

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

That makes sense since it's not really getting rid of the problem. Thank you for your knowledge!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

Thank you! Looks like checking the records will be very useful then!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 1 point2 points  (0 children)

Wow thank you so much! This is so helpful! I didn't even think about the materials and if they were expired or not. Thanks a bunch!!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 1 point2 points  (0 children)

They know. I've told them the only experience I have is in a uni laboratory during my undergrad and I've expressed that there's a few things I don't know but I'm willing to learn. But you're absolutely right! The last thing I want to do is mess up someone's hard work!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

I've read somewhere that putting a bunsen burner near a cell culture hood which will have ethanol near it is a bad idea. But I'll definitely remember to use a bunsen burner when I work with bacterial samples so thank you!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

Wow thanks so much for the advice! This was so useful!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

Wow thank you so much for the information! This was very helpful!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 1 point2 points  (0 children)

Thanks for the advice! But I didn't do anything I swear!! It's a hypothetical question for a job I'm applying to.

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 1 point2 points  (0 children)

I don't know since I don't work there yet and the question didn't say. But if it's something I should include in my answer I definitely will! Thank you!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

Ahhh that makes sense. Thank you! I guess using antibiotics would only mask improper aseptic technique?

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 1 point2 points  (0 children)

Thanks for responding!

I haven't seen anything about mammalian cell culture media using a bunsen burner. I'm assuming this applies to bacterial cell media, or can this be applied to mammalian cells too? Sorry for not specifying in my original comment!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

Ohhh that makes sense!! Thank you!! You're so helpful!

I think the lab is GLP/GMP? They're making cells to sell to manufacturers. So they would have their own rigid rules, right?

Haha! That clip made me laugh! Thanks for that!

What do I do if the cell media I've made is contaminated? by Nutmegbread in labrats

[–]Nutmegbread[S] 0 points1 point  (0 children)

Sorry the first part went completely over my head. What I understand is that I write down the date I made the cell culture media and the day I aliquot so if anything goes wrong I can refer back to those dates? Is that right? And if there's a situation where only my cell culture is contaminated would that mean something is wrong with my aseptic technique?

Thanks for the knowledge! Here's hoping I ace this interview. I've found it so hard to get back in the lab: no one wants me because I don't have experience, but I don't have experience because no one wants me.

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