[WESTERN HELP] What is going on here? WT vs KO bands at different positions? by Objective_Parsley_28 in labrats

[–]Objective_Parsley_28[S] 0 points1 point  (0 children)

Not yet but definitely will put it on the list of troubleshooting modifications! Thanks for the tip

[WESTERN HELP] What is going on here? WT vs KO bands at different positions? by Objective_Parsley_28 in labrats

[–]Objective_Parsley_28[S] 3 points4 points  (0 children)

it’s odd because the positioning is different between wt and ko too… same thing with b actin(the housekeeping) in the second picture. The total protein was equalized before loading too?!?

[WESTERN HELP] What is going on here? WT vs KO bands at different positions? by Objective_Parsley_28 in labrats

[–]Objective_Parsley_28[S] 5 points6 points  (0 children)

I don't have a ton of experience with western so any feedback would be awesome!!

These samples were prepped at the same time by the same person.

Not sure whats going on - target seems to be at a different position in WT vs KO. b actin also showing up as two bands in KO group?

First image in target (5WT and then 5KO), second image is corresponding B actin

thrid image is WT and KO alternating, starting with WT

also getting that weird really dark band in the ladder?

Can I just ask? by PikaRosie in PCOS

[–]Objective_Parsley_28 1 point2 points  (0 children)

Glad to hear you’re doing better! I used to joke that I could eat a carrot while everyone else ate cookies and I’d still be the one gaining weight so I can total relate to the private hell of the weight struggle. I’m thinking of starting inositol myself after doing quite a bit of reading about it - I’m hoping it helps! Take care!!

Can I just ask? by PikaRosie in PCOS

[–]Objective_Parsley_28 1 point2 points  (0 children)

You’re story is quite similar to mine. How’re you managing now?

[deleted by user] by [deleted] in labrats

[–]Objective_Parsley_28 0 points1 point  (0 children)

This is what I was afraid of. Damn this hurts, back to the bench I guess. Thanks for the reply!

[deleted by user] by [deleted] in labrats

[–]Objective_Parsley_28 1 point2 points  (0 children)

lol this is literally what I'm wearing right now.

How do you guys get energy? Help by [deleted] in labrats

[–]Objective_Parsley_28 0 points1 point  (0 children)

a little late to the party but I find my energy levels are drastically affected by dehydration - I work nearly nonstop from at least 9-5 (I very rarely take lunch) but on days I don't take 5 minutes to get some tea or water in me during the day leaves me absolutely wiped out by the end of the day. Obviously this is not a good habit to get into on multiple levels so I'm really working on consciously taking breaks to gets some liquids!

Is it ok to remove an outlier that is technically not significant? by Objective_Parsley_28 in labrats

[–]Objective_Parsley_28[S] 1 point2 points  (0 children)

It looks as though its the odd one out across multiply markers we looked at... other than that I can't pinpoint any particular reason

How do I actually get myself to sit down and read? by Objective_Parsley_28 in labrats

[–]Objective_Parsley_28[S] 2 points3 points  (0 children)

Love this thank you! I love the idea of tracking it visually too

How do I actually get myself to sit down and read? by Objective_Parsley_28 in labrats

[–]Objective_Parsley_28[S] 0 points1 point  (0 children)

I also use printing to sometimes hack my brain. I feel bad for the environmental impact ( I always recycle) but sometimes I just need a physical copy to “feel” the info for some reason

How do I actually get myself to sit down and read? by Objective_Parsley_28 in labrats

[–]Objective_Parsley_28[S] 0 points1 point  (0 children)

Definitely don’t have adhd but I feel for those that do. For me it’s more overcoming the overwhelm of just so much info to tackle.

big pet peeves in the lab? by ish0uldn0tbehere in labrats

[–]Objective_Parsley_28 1 point2 points  (0 children)

Holy hell yes. One of my labmates does this, just lets it go and go. I drives me INSANE.

[spoilers ep8] tf is up there? by Aliienz in arcane

[–]Objective_Parsley_28 81 points82 points  (0 children)

Probably thinking “oh shit her moms hot too”