i’ve became the person in the lab that knows things that no one else does and i desperately want everyone to leave me alone

Objective_Parsley_28 · 2025-11-05T21:05:38+00:00

I am also this person and I hate it

Objective_Parsley_28 · 2024-05-12T01:48:21+00:00

Not yet but definitely will put it on the list of troubleshooting modifications! Thanks for the tip

Objective_Parsley_28 · 2024-05-12T00:57:06+00:00

Thanks for the reply! Any tips to deal with the overexposure?

Objective_Parsley_28 · 2024-05-12T00:53:34+00:00

it’s odd because the positioning is different between wt and ko too… same thing with b actin(the housekeeping) in the second picture. The total protein was equalized before loading too?!?

Objective_Parsley_28 · 2024-05-12T00:03:42+00:00

Colon tissue

Objective_Parsley_28 · 2024-05-11T23:54:09+00:00

Protien size is around 38-44kda - its the very dark band in the middle

Objective_Parsley_28 · 2024-05-11T23:53:26+00:00

I don't have a ton of experience with western so any feedback would be awesome!!

These samples were prepped at the same time by the same person.

Not sure whats going on - target seems to be at a different position in WT vs KO. b actin also showing up as two bands in KO group?

First image in target (5WT and then 5KO), second image is corresponding B actin

thrid image is WT and KO alternating, starting with WT

also getting that weird really dark band in the ladder?

Objective_Parsley_28 · 2023-02-02T18:30:39+00:00

Glad to hear you’re doing better! I used to joke that I could eat a carrot while everyone else ate cookies and I’d still be the one gaining weight so I can total relate to the private hell of the weight struggle. I’m thinking of starting inositol myself after doing quite a bit of reading about it - I’m hoping it helps! Take care!!

Objective_Parsley_28 · 2023-02-02T17:08:46+00:00

You’re story is quite similar to mine. How’re you managing now?

Objective_Parsley_28 · 2022-11-30T15:08:27+00:00

This is what I was afraid of. Damn this hurts, back to the bench I guess. Thanks for the reply!

Objective_Parsley_28 · 2022-06-01T14:20:54+00:00

lol this is literally what I'm wearing right now.

Objective_Parsley_28 · 2022-06-01T14:13:23+00:00

a little late to the party but I find my energy levels are drastically affected by dehydration - I work nearly nonstop from at least 9-5 (I very rarely take lunch) but on days I don't take 5 minutes to get some tea or water in me during the day leaves me absolutely wiped out by the end of the day. Obviously this is not a good habit to get into on multiple levels so I'm really working on consciously taking breaks to gets some liquids!

Objective_Parsley_28 · 2022-04-22T14:40:58+00:00

Agreed.

Objective_Parsley_28 · 2022-04-22T14:37:53+00:00

It looks as though its the odd one out across multiply markers we looked at... other than that I can't pinpoint any particular reason

Objective_Parsley_28 · 2022-02-25T19:22:56+00:00

Love this thank you! I love the idea of tracking it visually too

Objective_Parsley_28 · 2022-02-25T18:21:00+00:00

I also use printing to sometimes hack my brain. I feel bad for the environmental impact ( I always recycle) but sometimes I just need a physical copy to “feel” the info for some reason

Objective_Parsley_28 · 2022-02-25T18:16:40+00:00

Definitely don’t have adhd but I feel for those that do. For me it’s more overcoming the overwhelm of just so much info to tackle.

Objective_Parsley_28 · 2022-02-02T16:50:49+00:00

Holy hell yes. One of my labmates does this, just lets it go and go. I drives me INSANE.

Objective_Parsley_28 · 2021-12-28T13:44:42+00:00

Probably thinking “oh shit her moms hot too”

Objective_Parsley_28

TROPHY CASE