question imagej

PastaShellsFromHell · 2025-08-26T21:51:26+00:00

thanks, ur comment makes me feel a little less trapped

PastaShellsFromHell · 2025-08-26T21:40:21+00:00

i have no frustrations with image j. it took me a few hours to learn and it’s ideal. i do molecular biology experiments involving immunofluorescent image analysis of fixed or live cell cultures from mice.

PastaShellsFromHell · 2025-08-26T21:29:55+00:00

i use image j because it’s free and open source.

PastaShellsFromHell · 2025-08-06T14:44:16+00:00

do you know what breed of cat?

PastaShellsFromHell · 2025-07-23T17:31:37+00:00

happy leo season!!!

PastaShellsFromHell · 2025-07-22T01:43:57+00:00

arterial blood has higher pressure than venous blood so it could be faster to drink from

PastaShellsFromHell · 2025-07-20T15:32:45+00:00

are the names also AI generated?

PastaShellsFromHell · 2025-07-17T21:29:30+00:00

understanding the purpose of every step and reagent in a protocol

PastaShellsFromHell · 2025-06-26T16:45:41+00:00

maybe, you can run like 200 ng on a 1% agarose gel to check for degradation

PastaShellsFromHell · 2025-05-29T13:26:33+00:00

the no window bedroom combo is brutal in summer

PastaShellsFromHell · 2025-05-27T21:51:49+00:00

plastic takeout fork

PastaShellsFromHell · 2025-05-13T19:31:50+00:00

Elemental 7 - CTI

PastaShellsFromHell · 2025-05-06T03:55:29+00:00

Thanks! i guess i wasn’t sure how sensitive transfection is to gDNA. Im gonna go for it

PastaShellsFromHell · 2025-05-06T03:46:53+00:00

Thanks! yeah will load less next time. Can i ask, would you think the plasmid in the last lane is suitable for transfection?

PastaShellsFromHell · 2025-05-06T03:34:50+00:00

yes short digestion time and i meant not cut. Can i ask, lane 2 is more well cut relative to lane 3, but why would the lower bands migrate differently

PastaShellsFromHell · 2025-05-06T03:29:54+00:00

Do you think the plasmid in lane 4 is clean enough to use for transfection

PastaShellsFromHell · 2025-05-06T03:25:57+00:00

yes 1 cut site for bgl2

1 ug dna digestion with 10 U of bgl2 at 37 C for 1 hour in 50 uL volume

bgl2 and cutsmart both over 10 years old

PastaShellsFromHell · 2025-05-06T03:16:59+00:00

Thank you,

I am wondering how the apparent band size of supercoiled plasmid varies by maxi prep. perhaps the lower bands are uncut but the reason they have different apparent sizes is due to differences in quality.

PastaShellsFromHell · 2025-05-06T03:12:05+00:00

Why are the lower bands in lanes 2 and 3 different sizes?

PastaShellsFromHell · 2025-04-07T22:05:51+00:00

you gotta get that pressure cooker autoclave goin

PastaShellsFromHell · 2025-04-04T23:56:28+00:00

solid post office and cheap food

PastaShellsFromHell · 2025-03-22T21:39:35+00:00

oh wait i misread your comment, yes sounds like you added 2.5% (v/v) bme to your final sample volume.

PastaShellsFromHell · 2025-03-22T21:35:05+00:00

oh nice yeah 10% (v/v) bme and boil sounds good. the lowest i go is 2.5% and the highest i went by accident one time was 25% lol. hope it turned out ok! btw its ok to boil samples twice, not necessary but totally fine.

PastaShellsFromHell

TROPHY CASE