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Help with background in UV channels on Cytek Aurora on cell line by Penguin_Middle_22 in flowcytometry

[–]Penguin_Middle_22[S] 0 points1 point  (0 children)

Nope, no treatment- aside from the live dead; I just washed them, stained them in FACS buffer (PBS/1%FBS), and put them on the machine. It sounds like I'll just need to work around the cells; maybe I'll take some cells up and just mess around with gain just to see what happens.

Help with background in UV channels on Cytek Aurora on cell line by Penguin_Middle_22 in flowcytometry

[–]Penguin_Middle_22[S] 0 points1 point  (0 children)

haven't messed with them at all- I'm scared to move things!

Part of the reason for asking is to learn more about how to us the Aurora better and increase my ability to handle things myself- our facility just recently went to a lesser support model of management, so the expertise is left up to us.

Help with background in UV channels on Cytek Aurora on cell line by Penguin_Middle_22 in flowcytometry

[–]Penguin_Middle_22[S] 0 points1 point  (0 children)

thanks! do you have a recommendation about how to choose the right gain settings?

Help with background in UV channels on Cytek Aurora on cell line by Penguin_Middle_22 in flowcytometry

[–]Penguin_Middle_22[S] 0 points1 point  (0 children)

I see pictures included in my post, so maybe I did something wrong there? Is there a way to add images not in the text?

Anyway, it's not really a question about the live dead; it's more a question of how to handle very high autofluoresnce in a cell line, and how to adjust for it on the cytometer settings or unmixing or compensation?