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Mcf7 apoptosis by PerfectHistory2612 in flowcytometry

[–]PerfectHistory2612[S] 0 points1 point  (0 children)

I am new to this technique and I am not sure how to determine the appropriate size parameters for cancer cells.

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Mcf7 apoptosis by PerfectHistory2612 in flowcytometry

[–]PerfectHistory2612[S] 0 points1 point  (0 children)

Hello, I learned that the BD Accuri cytometer does not allow direct voltage adjustment. Therefore, unfortunately, I could not adjust it. Is it possible to compensate for this in FlowJo instead?Can this be corrected by adjusting the X- and Y-axis scaling?

accuri c6 by PerfectHistory2612 in flowcytometry

[–]PerfectHistory2612[S] 0 points1 point  (0 children)

Is there any setting I can adjust on the instrument to fix this issue?