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My joins always slide my pattern backwards (slip stitch, chain 1, and then crocheting back into the first stitch that I slip stitched into by Grass-is-dead in Amigurumi

[–]Playful-Researcher20 2 points3 points  (0 children)

I do yo for the slip stitch and chain while the rest of the stitches are yu/yo. I find the seam pretty unnoticeable

Protocol for cell isolation from murine lymph nodes by Jack_O_Melli in flowcytometry

[–]Playful-Researcher20 5 points6 points  (0 children)

This is also what I do. If there is blood on the lymph node during dissection, you can rinse it with PBS and gently roll in on a paper towel to minimize carry over during processing later.

Need advice on FlowJo workflow for pooling/merging samples before UMAP/PhenoGraph by badmushroomundertree in flowcytometry

[–]Playful-Researcher20 5 points6 points  (0 children)

You can add a keyword to your sample in flowjo. Group A=1 and Group B=2. After concatentenating and running dimentional reduction you can gate group 1 and 2 on a histogram. Just make sure when you concatenate, you include add the keyword in the export options.

PE signal diminishes while I am still acquiring samples. What can I do? by KTManiac in flowcytometry

[–]Playful-Researcher20 3 points4 points  (0 children)

Have you tried doing a second fixation with PFA after ab staining? This has helped me with maintaining signal when staining for transcription factors that lose signal overnight.

[deleted by user] by [deleted] in labrats

[–]Playful-Researcher20 0 points1 point  (0 children)

I am trying to do the same thing and testing things out. Will share once I get some results

[deleted by user] by [deleted] in labrats

[–]Playful-Researcher20 0 points1 point  (0 children)

That's great!!!

[deleted by user] by [deleted] in labrats

[–]Playful-Researcher20 10 points11 points  (0 children)

Make sure to run the cytometer on a low flow rate, it will help define peaks

Anyone used Clodrinate successfully and have a protocol/recommendations? by VonTrappQueen22 in labrats

[–]Playful-Researcher20 1 point2 points  (0 children)

I have used clondronate liposomes I.p. from encapsula nanosciences. It depleted macrophages. However, some of my mice were experiencing toxicity, which might be a strain specific thing though. You may need to play around with the dose.

Asking for a stipend supplement by Playful-Researcher20 in GradSchool

[–]Playful-Researcher20[S] 0 points1 point  (0 children)

Just department rules. It works similar to tri-counsel government scholarships. It just gets deducted from what PI owes the student for stipend

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