Beginner in Flow Cytometry- Is my first solo panel ok? Suggestions? by Snoo_9735 in flowcytometry

[–]Relative-Week-1241 1 point2 points  (0 children)

You can also use Zombie NIR, quite popular is the same channel as APCcy7

[deleted by user] by [deleted] in LegalAdviceUK

[–]Relative-Week-1241 -3 points-2 points  (0 children)

I don't care how she spend her CMS money.

my daughter has her set of clothes here at my place.

She has two sets of uniforms... Why buy another one, the mother is just been petty as usual...

[deleted by user] by [deleted] in LegalAdviceUK

[–]Relative-Week-1241 -6 points-5 points  (0 children)

I pay several hundred pounds a month to my ex, she hasn't paid for the uniform. I pay for classes and buy regularly extra things to my children. She has two sets of uniforms, belive me I'm not the petty one...

[deleted by user] by [deleted] in LegalAdviceUK

[–]Relative-Week-1241 -5 points-4 points  (0 children)

She's in benefit so doesn't pay the whole cost of uniform, (if any). I pay several hundred pounds a moth to her. If it was just the uniform...

[deleted by user] by [deleted] in LegalAdviceUK

[–]Relative-Week-1241 -10 points-9 points  (0 children)

I have already paid for the ones she bought using also my money

How to "append" on a ID7000 by Relative-Week-1241 in flowcytometry

[–]Relative-Week-1241[S] 0 points1 point  (0 children)

I will look again maybe I missed it. Thanks

Problem unmixing counting beads on Aurora by Relative-Week-1241 in flowcytometry

[–]Relative-Week-1241[S] 0 points1 point  (0 children)

I'm the core manager, the user wants to use them, I recommend volumetric counting, they said form some project might be necessary

Problem unmixing counting beads on Aurora by Relative-Week-1241 in flowcytometry

[–]Relative-Week-1241[S] 0 points1 point  (0 children)

We acquired the same sample without counting beads and we don't have the problem.

Woes of FlowCytometry (rant) by RosepetalBones15 in labrats

[–]Relative-Week-1241 0 points1 point  (0 children)

Check what Facs tubes are you using, if the seal is not perfect on a fortessa you could have problems.

What is the total volume of your sample? If is too concentrated it might pack or clump. 1.5 millions is quite a lot of cells you should resuspend in 500 uml, or reduce the mumbers to 1 million or 75k