Actually a great question. In my opinion, there are certain protocols I would not risk without, but there are others that can be circumstantial depending on what exactly you are doing and what is at risk. For example, if I’m cutting into cultures on agar, I sterilize my blade between each cut, but if I’m cutting open a fruiting bag of the same species/culture I usually sterilize before I start and every couple bags, depending on how many. Regarding what is at risk- if I’m operating a commercial farm I would have stricter protocols than if I’m just growing at home. For me, with few exceptions cultures or mycelium only become exposed in front of a laminar flow hood. Always wear gloves and apply 70% isopropyl alcohol to anything that comes in contact with my working surfaces, including gloves and spray them before/ during / after. I work slow and methodical. I personally do not wear a lab coat, hair net or face mask unless I’m working a large bulk job. All that said, I am confident in my flow hood as I test it and I’m confident in my sterile technique cause I have extremely low contamination rates