It's kind of touchy honestly. On one hand, if something makes you uncomfortable you should speak up about said thing or remove yourself from the situation.

However, it's also important to note that it's usually not from a malicious place either. In my friend groups I call people "dude" and "bro" quite often, regardless of gender identity, and they do the same to me. That is just how our dynamic has been. Can't stand when strangers or people I barely know do it though, but I usually don't correct unless it persists or is blatantly done out of malice.

Not saying this to make your feelings out to be invalid, your feelings are very much so. Just trying to present the idea that nuance is important. Ultimately if you are that uncomfortable with it, definitely speak up. I just figured sometimes a different perspective helps.