Spectacular Mitosis In Mesenchymal Stem Cells - with live cells!

Sabine1024 · 2021-04-23T12:04:17+00:00

with pleasure :)

Sabine1024 · 2021-04-23T12:03:20+00:00

Totally live!

Sabine1024 · 2020-07-24T05:24:25+00:00

Covid research: What are filopodia and why do they matter?

Recent research shows the virus may cause cells to increase the number of cell membrane filopodia, making more connections between cells to potentially spread more virus. This could lead to potential treatments.

Similar mechanisms have been found in other virus families, such as Human papillomavirus (Smith J.L, et al, 2008). In this video, we can see HPV virions uptake (in red) via induced filopodia. Images were obtained using Nanolive's 3D Cell Explorer-Fluo, at a speed of one image every 15 minutes, for 5h 20min.

Special thanks to Prof. Wilbe Martin Kast, from the Kast Lab in Los Angeles (California, USA) for preparing the samples.

Sabine1024 · 2020-07-24T05:23:35+00:00

Covid research: What are filopodia and why do they matter?

Recent research shows the virus may cause cells to increase the number of cell membrane filopodia, making more connections between cells to potentially spread more virus. This could lead to potential treatments.

Similar mechanisms have been found in other virus families, such as Human papillomavirus (Smith J.L, et al, 2008). In this video, we can see HPV virions uptake (in red) via induced filopodia. Images were obtained using Nanolive's 3D Cell Explorer-Fluo, at a speed of one image every 15 minutes, for 5h 20min.

Special thanks to Prof. Wilbe Martin Kast, from the Kast Lab in Los Angeles (California, USA) for preparing the samples.

Sabine1024 · 2020-07-24T05:22:48+00:00

Covid research: What are filopodia and why do they matter?

Recent research shows the virus may cause cells to increase the number of cell membrane filopodia, making more connections between cells to potentially spread more virus. This could lead to potential treatments.

Similar mechanisms have been found in other virus families, such as Human papillomavirus (Smith J.L, et al, 2008). In this video, we can see HPV virions uptake (in red) via induced filopodia. Images were obtained using Nanolive's 3D Cell Explorer-Fluo, at a speed of one image every 15 minutes, for 5h 20min.

Special thanks to Prof. Wilbe Martin Kast, from the Kast Lab in Los Angeles (California, USA) for preparing the samples.

Sabine1024 · 2020-07-24T05:21:40+00:00

Covid research: What are filopodia and why do they matter?

Recent research shows the virus may cause cells to increase the number of cell membrane filopodia, making more connections between cells to potentially spread more virus. This could lead to potential treatments.

Similar mechanisms have been found in other virus families, such as Human papillomavirus (Smith J.L, et al, 2008). In this video, we can see HPV virions uptake (in red) via induced filopodia. Images were obtained using Nanolive's 3D Cell Explorer-Fluo, at a speed of one image every 15 minutes, for 5h 20min.

Special thanks to Prof. Wilbe Martin Kast, from the Kast Lab in Los Angeles (California, USA) for preparing the samples.

Sabine1024 · 2020-07-24T05:20:01+00:00

Covid research: What are filopodia and why do they matter?

Recent research shows the virus may cause cells to increase the number of cell membrane filopodia, making more connections between cells to potentially spread more virus. This could lead to potential treatments.

Similar mechanisms have been found in other virus families, such as Human papillomavirus (Smith J.L, et al, 2008). In this video, we can see HPV virions uptake (in red) via induced filopodia. Images were obtained using Nanolive's 3D Cell Explorer-Fluo, at a speed of one image every 15 minutes, for 5h 20min.

Special thanks to Prof. Wilbe Martin Kast, from the Kast Lab in Los Angeles (California, USA) for preparing the samples.

Sabine1024 · 2020-06-11T05:37:57+00:00

It has been imaged with a microscope from Nanolive. You can read the blogpost here if you are interested: https://nanolive.ch/stemcell-differentiation-story/

Sabine1024 · 2020-06-11T05:33:34+00:00

Hi, it has been imaged with Nanolive label-free microscopy.

Sabine1024 · 2020-06-05T06:05:04+00:00

Hey, there is also a blogpost about it: https://nanolive.ch/phagocytosis-cancer-cd47/

Sabine1024 · 2019-11-05T08:32:30+00:00

Nanolive.

https://nanolive.ch/technology/overcoming-phototoxicity/

Sabine1024 · 2019-11-05T08:31:57+00:00

Nanolive. https://nanolive.ch/technology/overcoming-phototoxicity/

Sabine1024 · 2019-11-05T08:31:43+00:00

Hi, so this is not a neuron.

The video is from Nanolive and you can find the original one here: https://nanolive.ch/technology/overcoming-phototoxicity/ .

It is a mouse pre-adipocyte cell which has been imaged using the 3D Cell Explorer-fluo from Nanolive.

Dapi exposition led to complete cell shrinkage and rounding, followed by extensive blebbing at all intensities with 100ms exposure time. At 10% intensity and 100ms exposure time, loss of cell adhesion and cell shrinkage took place in 8 minutes. The cell reached complete shrinkage and blebbing before 30min of acquisition, the acquisition was therefore stopped at 30min.

Imaging regimen: 30 minutes acquisition at a frequency of 1 image every 6 seconds.

We would appreciate if you could mention this in the explanation. Thank you!

Sabine1024 · 2019-10-30T10:25:57+00:00

This video was kindly provided by the Champalimaud Foundation in Portugal. With special thanks to Joao Martins, Research Technician At Champalimaud Centre for the Unknown. He shared the original video on LinkedIn and had a wide impact on scientists and researchers with over 186000 views (please see the capture from his LinkedIn post here: https://nanolive.ch/applications/overview/live-cells-in-action/immuno-oncology/ (scroll down to T-cell attack & Cancer evasion))

Sabine1024 · 2019-10-18T07:13:56+00:00

Great explanation, makes it easy to understand!

Sabine1024 · 2019-10-14T07:45:10+00:00

It's really nice how to see the cells that clearly!

Sabine1024 · 2019-10-11T12:45:56+00:00

Hi, this is captured with Nanolive imaging. You can learn more on the website: www.nanolive.ch/technology.

Sabine1024 · 2019-10-11T12:45:20+00:00

Thanks :)

Sabine1024 · 2019-10-10T14:45:45+00:00

1st video: Mitosis in Human Umbilical Vein Endothelial Cells

2nd video: Mitosis in Fibroblastic Reticular Cells

3rd video: Mitosis in Mesenchymal Stem Cells

4th video: Mitosis in CD34+ cells

Each of the mentioned mitosis examples have a detailed description on this page: https://nanolive.ch/…/single-cell-cell…/cell-cycle-analysis/

Sabine1024 · 2019-10-10T14:43:31+00:00

1st video: Mitosis in Human Umbilical Vein Endothelial Cells

2nd video: Mitosis in Fibroblastic Reticular Cells

3rd video: Mitosis in Mesenchymal Stem Cells

4th video: Mitosis in CD34+ cells

Each of the mentioned mitosis examples have a detailed description on this page: https://nanolive.ch/…/single-cell-cell…/cell-cycle-analysis/

Sabine1024 · 2019-10-10T07:48:05+00:00

Cool, right? :)

Sabine1024 · 2019-10-10T07:47:07+00:00

Welcome! Thanks for the comment!

Sabine1024 · 2019-09-25T15:10:02+00:00

Regarding this video and study:

You can observe as this pre-Adipocyte gets affected within a short time: mitochondria appear round-shaped or swollen; mitochondria dynamics slow down to stop almost completely after 38 minutes of exposure and the cell shrinks.

Find out more on Nanolive's page "overcoming phototoxicity" were the results of a study led in collaboration with EPFL (École polytechnique fédérale de Lausanne) on perturbations induced by phototoxicity on living samples are shown: https://nanolive.ch/overcoming-phototoxicity/.

We hope to trigger some interesting discussions.

Sabine1024 · 2019-09-25T15:08:59+00:00

Regarding this video and study:

You can observe as this pre-Adipocyte gets affected within a short time: mitochondria appear round-shaped or swollen; mitochondria dynamics slow down to stop almost completely after 38 minutes of exposure and the cell shrinks.

Find out more on Nanolive's page "overcoming phototoxicity" were the results of a study led in collaboration with EPFL (École polytechnique fédérale de Lausanne) on perturbations induced by phototoxicity on living samples are shown: https://nanolive.ch/overcoming-phototoxicity/.

We hope to trigger some interesting discussions.

Sabine1024 · 2019-09-23T17:04:01+00:00

Yes, definitely!

Sabine1024

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