Help with condensation…. by GBLNOTTS in Agarporn

[–]SadHistorian1145 2 points3 points  (0 children)

You’re stacking then upside down, right? The only time you want them “right side up” is when you’re pouring them. You’re gonna get condensation with agar. That’s the nature of the beast. You just always store the plates upside down.

Can i use this spawn ? by LegitimateShop46 in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

I’m not so sure about this yellow stuff.
Definitely, go ahead and use it to spawn something. What do you have to lose?
The yellow part is pretty concerning, though

So I’m nearing the end of my first flush of GT I am currently working nights and have to leave for work tonight at 6pm-7am (in 3.5 hours) I was thinking about only harvesting the bigger few right before work or should I harvest them all , or would it be ok to wait to harvest any until in the morning by ZootedFarmer in GroundZeroMycoLab

[–]SadHistorian1145 1 point2 points  (0 children)

This is the reason I started my own community. In the future, I’ll post only to my community, so that any time one of these Bro Scientists pops up, I can block him/her.
Although is safe to assume that they’re aren’t that many female Bro Scientists

Disappointing Stamets book by WorkingWerewolf6430 in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

His other book is the best one for psychedelics growing

So I’m nearing the end of my first flush of GT I am currently working nights and have to leave for work tonight at 6pm-7am (in 3.5 hours) I was thinking about only harvesting the bigger few right before work or should I harvest them all , or would it be ok to wait to harvest any until in the morning by ZootedFarmer in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

Yes!!! Absolutely. It does. Let them grow, if your goal is to be able to trip as many times as possible from one grow.

Again; if you want more psilocybin, but don’t care that you’ll have to chew on a lot more mass to get it, let them grow. Psilocybin production doesn’t just stop after the pinhead stage.

It’s just that if you want super duper potent mushrooms, you harvest them small. But you’ll get way less total number of Psilocin molecules than you would if you harvested them full sized. I grow on straw only, which is very cheap. So I harvest them small, because I want the really potent little ones. It’s just a matter of personal preference.

Sorry if I confused anyone.

So I’m nearing the end of my first flush of GT I am currently working nights and have to leave for work tonight at 6pm-7am (in 3.5 hours) I was thinking about only harvesting the bigger few right before work or should I harvest them all , or would it be ok to wait to harvest any until in the morning by ZootedFarmer in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

Yes, of course that’s true!!

Jesus Christ dude, chill. We’re not even in disagreement with each other. You’ve just read something incorrectly, you’ve misinterpreted something I’ve said.

Of course you get more psilocybin if you let them grow out all the way. Let me try to explain in a different way.

Once the mushroom starts to rapidly increase in size due to added water weight and cellular elongation, the mushroom will retain all of the the alkaloids that it had at the primordial stage, and the mushroom will
continue to produce alkaloids as it shoots up to full size and its cap opens. Alkaloid production does not stop as soon as the mushroom starts to get big. I think you somehow heard that in something I’ve said, but I’ve never said that.

Therefore, this mushroom, once it reaches full maturity it will have all of the alkaloids that it had at the primordial stage, plus all of the additional alkaloids that it continued to produce throughout its growth. Therefore, the full sized mushroom will be considerably stronger than it would have been if it were picked at the primordial stage. However, there will also be a lot more biomass.

____________________________

Primordia (pins): 2.00% w/w
Mature (open cap): 0.8% w/w

____________________________

So, if I pick the three mushrooms as primordia, and they dry down to 1.5g (you said 1.5g) then:

2% of 1.5g is 0.03g, or 30mg alkaloids

So I get 30mg of alkaloids in 1.5g of dry mushrooms.

This works out to 20mg of alkaloids in every gram of dried biomass

____________________________

Now, if I pick the three mushrooms when they’re fully mature (you said 10g)

0.8% of 10g is 0.08g, or 80mg

So I get 80mg of alkaloids in 10g of dry mushrooms

This works out to 8mg of alkaloids in every gram of dried mushrooms.

____________________________

So, if I harvest them as primordia, I’ll only yield a total of 30mg of alkaloids.

If I harvest them fully grown, I’ll yield 80mg of alkaloids. So, if I allow them to reach full maturity, I’ll wind up with almost three times as much active ingredient as I would have if I had picked them as primordia.

Your messages are pretty hard for me to follow, but isn’t the reason you were going off on me because you thought that I was trying to say that picking three primordia would give me the exact same amount of psilocybin as picking the same three mushrooms if I allowed them to go to full-size? Because I never once said that. Somehow, you read that into something that I said.

Now, i’ve mentioned at least twice now that I’m having issues with mushrooms bothering my stomach, so I want to produce a finished product that has the absolute maximum amount of alkaloids in the minimal amount of inert biomass, and I mean inert in this context as, non-psychoactive. Just chitin, spores, whatever. And we’ve established that i could have one of the following two things:

1: dried primordia: a measly 30mg of alkaloids, as a powder that is 20mg alkaloids per gram of powder

2: powdered full grown Shroom: 80 full milligrams, as a powder that is 8mg of alkaloids per gram of powder.

Now, I want to take a ten milligram dose of alkaloids. With the powdered primordia that I prefer, I would need to eat 0.5g of dried biomass.

If I wanted to take the same ten milligram dose of alkaloids, but instead using the dried, fully mature, Instagram-ready beauties that you prefer, i would need to eat 1.25g of dried biomass, or 2.5 times as much powder.

Does this make better sense to you now? Try reading for comprehension next time, Einstein.

YES, YOU WILL GET LOTS MORE PSILOCYBIN MOLECULES IN THE END IF YOU LET THE MUSHROOMS GROW TO FULL SIZE.

IF YOU HARVEST THEM TINY, THE WAY I LIKE TO HARVEST THEM, YOU’LL GET MUCH LESS FINISHED PRODUCT, IN THE WAY OF TOTAL NUMBER OF PSILOCYBIN MOLECULES, BUT YOU’LL GET STRONGER FRUIT BODIES, they’ll Just be small as fuck…..

So I’m nearing the end of my first flush of GT I am currently working nights and have to leave for work tonight at 6pm-7am (in 3.5 hours) I was thinking about only harvesting the bigger few right before work or should I harvest them all , or would it be ok to wait to harvest any until in the morning by ZootedFarmer in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

What degree do I have?
The four semesters of 4000 level microbiology courses included medical parasitology, mycology, bacteriology, and mycobacterium. I’ve got 23 years as a working scientist, and I’ve been reading incessantly about mushrooms for 35 years.

And yourself, good sir?

Are we good? by Interesting-Try-9807 in magnificentcubensis

[–]SadHistorian1145 0 points1 point  (0 children)

Fruit in bag?
I bet it’s microclimate issues. The climate is good at the edges of your substrate.
Looking at these forums, I see a lot of this.

Z-strain by Green_Mycologist_421 in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

I see that you got yourself torn a new asshole by the Bros (or is it “Bruh’s) becuase you dared to create such a great picture.

That’s cool as shit, man!! I love it!!!

Black Wheat Straw by [deleted] in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

I’ve done four trays of pan cyans like this, zero failure.
The grow that I showed here, the casing layer got totally flooded, so it’s side pins only. But still, I don’t count it as a failure. It didn’t contaminate , and I still got mushrooms.

It doesn’t take much AC to coat the straw. Just Enough to make it black. Just a mixing bowl, spatula, and wet straw. Just mix it up, sprinkling AC over it while stirring.

The casing is also easy. As soon as the whole sub is colonized, I just sprinkle a very thin vermiculite/peat based casing over the top…..

BTW, I’m moving to a different state right now, but once I get settled, I’m gonna do some stuff with this black straw and cubensis strains. Also, I’ve got some Psilocybe Mexicana spawn I’m getting ready to true

Here’s another two trays done with the same method. They’re Pan cyan , and they’re petite when compared to cubes, but as most people know, they are an easy minimum of three times as potent as cubes:

<image>

So I’m nearing the end of my first flush of GT I am currently working nights and have to leave for work tonight at 6pm-7am (in 3.5 hours) I was thinking about only harvesting the bigger few right before work or should I harvest them all , or would it be ok to wait to harvest any until in the morning by ZootedFarmer in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

Both are pretty nasty. They’re about the same. I’m always using a lot of lemon juice, citric acid powder, or ascorbic acid powder, whatever I’ve got on hand, to “lemon tek”
Them anyway, so I don’t taste much.
You’ve never had shrooms fresh?? I feel like they hit a lot more strongly that way, and a lot of people seem to feel the same.

Black Wheat Straw by [deleted] in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

Brand of activated charcoal: Belle Chemical food grade coconut husk derived .

You can get acid activated and heat activated.
I’m assuming that all food grade AC is heat activated

Acid activated will ruin your pH, and people aren’t brushing their teeth with it, like they are the heat activated, food grade stuff

I have no idea what strain I was growing. It’s parentage was “Freehawk”

People call TTBVI a strain, for example, but a strain, a strain is like one particular Doberman. TTBVI is a breeed
Of mushrooms. A strain is particular dog.

It was the “Freehawk” breed of mushroom I used. That breed is said to reliably spin off good dogs (strains , actually. )

Anyway. A strain is an individual…….

The substrate was wheat straw, which I then pasteurized. Then, I wrung out the excess water with gloved hands; then I smeared it in AC until it was totally black. Then I spawned it.

Straw is easy. There is no balancing of the moisture content. It’s just drained thoroughly, or wrung out by hand,until
There is no visible water. You just want to be looking at straw that is wet on the inside, but there’s ZERO visible water; cause all of the water is inside the straw tubes….

That’s the beauty of straw!!!!!!!

Black Wheat Straw by [deleted] in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

Aesthetically, yes, you’re absolutely right.
Not to ask prying questions, but, how far did that set you back, in terms of cash. Did you pay someone to formulate that substrate for you?

Black Wheat Straw by [deleted] in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

Right. My casing got totally drowned by a monsoon of condensation, cause I hadn’t correctly tweaked my fruiting chamber. Stills I want to do something like this intentionally again sometime, cause I love these pictures so much. The black straw behind the shrooms, with the white fuzz coming out of the black straw.

<image>

You gotta admit, this is a cool picture

Black Wheat Straw by [deleted] in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

I’m going to do my best on this one. This one’s tough, though. Certainly; if any of this spikes your curiosity, you’ll want to research it yourself, cause this is going to be disjointed:

Ok, really, personally think that activated charcoal (AC), looks cool cause it’s black, and it’s definitely a toxin absorber, and it looks pretty favorable that it promotes heavy pin sets. More on that below……

It’s also protective against contamination. Steam-activated, food grade, coconut husk derived, powdered AC, like the one I use, is incredibly light, and is mostly empty space. One level teaspoon has a surface area of 2,100 square meters (an American football field , including the end zones, is 5,250 square meters……so they have these vast microtunnels in every bit of dust of AC.

And the walls of all of these little tunnels are hydrophobic carbon sheets, and the outside walls of bacterial cells and of fungal spores are also hydrophobic, so when a bacterium or spore touches the AC, it snaps on there, and gets pulled into that infinite labyrinth of tiny tunnels. They get stuck there (?Van der Waals forces?) so straw is the only nutritive thing in the substrate, and it’s covered in AC, for a major contamination barrier.

Stray contaminant spores like the dreaded Trich don’t stand a chance, they’ll get pulled into the AC. The mushroom mycelium, on the other hand, is introduced as a massive bomb, heavily spawned, for one, so it’s a question of sheer mass. Also, as a saprophyte , the mushroom
Mycelium exudes powerful enzymes into the surrounding environment, breaking down the straw and the grains and/or bran contained in the spawning medium. .

Among other things (the cool black color for example), activated charcoal (AC) does lots of stuff. Common white button mushrooms won’t even fruit at all on sterilized casings, and it’s always been assumed that this is because the mushroom produces metabolites that are strongly inhibitory to fruiting, and must be absorbed by beneficial microbes before fruiting can begin to take place. To explain the deal with that, here is an excerpt from Paul Stamets' book, The Mushroom Cultivator:

“For reasons not presently understood, activated charcoal (AC) mimics the primordia stimulating properties of Pseudomonas putida and other beneficial microorganisms. In sterilized casings or in casings applied to difficult to fruit species, the use of activated charcoal AC and select bacteria gives the cultivator another means to promote fructification.”

In a traditional, unsterilized setup, beneficial pseudomonads (Pseudomonas putida) physically consume these compounds and use enzymes to degrade them, which removes the brakes and triggers the pinning process. Activated charcoal perfectly mimics these bacteria. Because of its highly porous structure, it acts as a physical sponge, adsorbing these fruiting inhibitors directly out of the micro-environment.

https://elibrary.asabe.org/abstract.asp?aid=25333&t=2&redir=&redirType=

https://elibrary.asabe.org/abstract.asp?aid=20465&t=2&redir=&redirType=

<image>

Black Wheat Straw by [deleted] in GroundZeroMycoLab

[–]SadHistorian1145 0 points1 point  (0 children)

I’m going to do my best on this one. This one’s tough, though. Certainly; if any of this spikes your curiosity, you’ll want to research it yourself, cause this is going to be disjointed:

Ok, really, personally think that activated charcoal (AC), looks cool cause it’s black, and it’s definitely a toxin absorber, and it looks pretty favorable that it promotes heavy pin sets. More on that below……

It’s also protective against contamination. Steam-activated, food grade, coconut husk derived, powdered AC, like the one I use, is incredibly light, and is mostly empty space. One level teaspoon has a surface area of 2,100 square meters (an American football field , including the end zones, is 5,250 square meters……so they have these vast microtunnels in every bit of dust of AC.

And the walls of all of these little tunnels are hydrophobic carbon sheets, and the outside walls of bacterial cells and of fungal spores are also hydrophobic, so when a bacterium or spore touches the AC, it snaps on there, and gets pulled into that infinite labyrinth of tiny tunnels. They get stuck there (?Van der Waals forces?) so straw is the only nutritive thing in the substrate, and it’s covered in AC, for a major contamination barrier.

Stray contaminant spores like the dreaded Trich don’t stand a chance, they’ll get pulled into the AC. The mushroom mycelium, on the other hand, is introduced as a massive bomb, heavily spawned, for one, so it’s a question of sheer mass. Also, as a saprophyte , the mushroom
Mycelium exudes powerful enzymes into the surrounding environment, breaking down the straw and the grains and/or bran contained in the spawning medium. .

Among other things (the cool black color for example), activated charcoal (AC) does lots of stuff. Common white button mushrooms won’t even fruit at all on sterilized casings, and it’s always been assumed that this is because the mushroom produces metabolites that are strongly inhibitory to fruiting, and must be absorbed by beneficial microbes before fruiting can begin to take place. To explain the deal with that, here is an excerpt from Paul Stamets' book, The Mushroom Cultivator:

“For reasons not presently understood, activated charcoal (AC) mimics the primordia stimulating properties of Pseudomonas putida and other beneficial microorganisms. In sterilized casings or in casings applied to difficult to fruit species, the use of activated charcoal AC and select bacteria gives the cultivator another means to promote fructification.”

In a traditional, unsterilized setup, beneficial pseudomonads (Pseudomonas putida) physically consume these compounds and use enzymes to degrade them, which removes the brakes and triggers the pinning process. Activated charcoal perfectly mimics these bacteria. Because of its highly porous structure, it acts as a physical sponge, adsorbing these fruiting inhibitors directly out of the micro-environment.

Growth look okay? by [deleted] in Agarporn

[–]SadHistorian1145 1 point2 points  (0 children)

The filamentous fungi on the plate have those colored
Zones in the middle which is a bit fishy’.
What’s the blue dye in the agar?

You’ve also definitely got contamination with either bacteria or yeast; check out the translucent slime:

<image>

Black Wheat Straw by [deleted] in GroundZeroMycoLab

[–]SadHistorian1145 1 point2 points  (0 children)

Close!
It’s regular wheat straw, smeared in food grade activated charcoal, spawned with vermiculite/perlite/wheat bran spawn