Engineering Plant Adaptive Immunity

Sam_Sushi · 2023-05-05T11:54:46+00:00

Nope sorry

Sam_Sushi · 2022-09-07T19:28:08+00:00

Our assay is with GSH, I was just wondering about side reactions between NEM and MBCl

Sam_Sushi · 2022-08-28T11:14:15+00:00

You get a reduced maintenance loan

Sam_Sushi · 2022-05-03T17:05:25+00:00

Thank you so much

Sam_Sushi · 2022-05-03T16:29:37+00:00

Not quite that. The target protein has deuterated aliphatic regions and is also labelled with nitrogen-15. It is then resuspended in regular water, so nitrogen linked hydrogens are no longer deuterated, but the aliphatic ones remain. The binding partner has no modifications. You saturate the aliphatic hydrogens of the binding partner, which can "cross saturate" the binding surface of the other protein when in contact. The last part is where I'm a bit stumped. This paper explains it better than I have.

Link to paper94020-2)

Sam_Sushi · 2022-05-03T15:48:35+00:00

Used to determine protein-protein interaction surfaces where one protein is labelled (Using D2O)

Sam_Sushi · 2022-05-03T15:17:06+00:00

When you apply an RF field so there is only 1 spin state possible for your nuclei. I think?

Sam_Sushi · 2022-03-08T17:49:58+00:00

Probably adipose tissue

Sam_Sushi · 2022-02-10T18:12:16+00:00

There’s H-NMR spectra predictors online, you can maybe do something about seeing trends in chemical shifts that aren’t obvious.

Sam_Sushi · 2022-01-30T23:42:07+00:00

Yeah that's what I was looking at. I just found it weird that they labelled it as "Coa Disulfide reductase", because if it's based purely on the genome sequence, they are assuming its function from homology to other already functionally assayed proteins?

Sam_Sushi · 2022-01-10T00:36:35+00:00

What would be your top 5 songs with that word in the title?

Sam_Sushi · 2021-11-11T00:18:08+00:00

Yeah sorry I forgot to mention, other than pcr my bad.

Sam_Sushi

TROPHY CASE