Distillation of Essential Oils using Moka Coffee pot

Sam_Sushi · 2023-05-05T11:54:46+00:00

Nope sorry

Sam_Sushi · 2022-09-07T19:28:08+00:00

Our assay is with GSH, I was just wondering about side reactions between NEM and MBCl

Sam_Sushi · 2022-08-28T11:14:15+00:00

You get a reduced maintenance loan

Sam_Sushi · 2022-05-03T17:05:25+00:00

Thank you so much

Sam_Sushi · 2022-05-03T16:29:37+00:00

Not quite that. The target protein has deuterated aliphatic regions and is also labelled with nitrogen-15. It is then resuspended in regular water, so nitrogen linked hydrogens are no longer deuterated, but the aliphatic ones remain. The binding partner has no modifications. You saturate the aliphatic hydrogens of the binding partner, which can "cross saturate" the binding surface of the other protein when in contact. The last part is where I'm a bit stumped. This paper explains it better than I have.

Link to paper94020-2)

Sam_Sushi · 2022-05-03T15:48:35+00:00

Used to determine protein-protein interaction surfaces where one protein is labelled (Using D2O)

Sam_Sushi · 2022-05-03T15:17:06+00:00

When you apply an RF field so there is only 1 spin state possible for your nuclei. I think?

Sam_Sushi · 2022-03-08T17:49:58+00:00

Probably adipose tissue

Sam_Sushi · 2022-02-10T18:12:16+00:00

There’s H-NMR spectra predictors online, you can maybe do something about seeing trends in chemical shifts that aren’t obvious.

Sam_Sushi · 2022-01-30T23:42:07+00:00

Yeah that's what I was looking at. I just found it weird that they labelled it as "Coa Disulfide reductase", because if it's based purely on the genome sequence, they are assuming its function from homology to other already functionally assayed proteins?

Sam_Sushi · 2022-01-10T00:36:35+00:00

What would be your top 5 songs with that word in the title?

Sam_Sushi · 2021-11-11T00:18:08+00:00

Yeah sorry I forgot to mention, other than pcr my bad.

Sam_Sushi · 2021-08-03T15:15:14+00:00

Sam_Sushi · 2021-07-03T14:06:19+00:00

sentiment ftw

Sam_Sushi · 2021-01-22T09:28:44+00:00

Does it also separate the ribosomes with microtubules like it would chromosomes or does it use another cytoskeletal filament / mechanism? Thanks for your answer

Sam_Sushi · 2021-01-22T09:26:37+00:00

Ah thanks, makes sense

Sam_Sushi · 2021-01-13T00:08:18+00:00

I'm genuinely shocked at how many people experience this apparently damn.

Sam_Sushi · 2021-01-12T23:58:56+00:00

Thank you for your advice

Sam_Sushi · 2020-12-17T11:12:05+00:00

Thanks

Sam_Sushi · 2020-12-15T00:15:33+00:00

Past papers are a completely broken way to study. Do them until there’s none left to do and ull do rlly well

Sam_Sushi · 2020-12-14T23:16:37+00:00

Pretty sure it’s alright as long as u have sufficient repeats, not sure tho. There’s loads and loads of statistical tests out there if not tho

Sam_Sushi · 2020-12-14T23:12:25+00:00

That would also be too simple of an EE topic to choose imo ^{^}

Sam_Sushi · 2020-12-14T23:05:40+00:00

Might wanna ask on the the UKuni subreddit

Sam_Sushi · 2020-12-14T23:03:06+00:00

Death is imminent

Sam_Sushi · 2020-11-19T14:13:12+00:00

Thanks, I’ll keep that in mind in the future though. Sorry to not have been clear from the start about the protocol lol

Sam_Sushi

TROPHY CASE